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重组RabMAb

重组Anti-G3BP2 (isoform a)抗体[EPR27325-43] - BSA and Azide free (ab317688)

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Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Flow Cytometry (Intracellular) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
  • Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR27325-43] to G3BP2 (isoform a) - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-G3BP2 (isoform a)抗体[EPR27325-43] - BSA and Azide free
    参阅全部 G3BP2 (isoform a) 一抗

  • 描述

    兔单克隆抗体[EPR27325-43] to G3BP2 (isoform a) - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    This antibody specifically recognizes G3BP2 (isoform a) but not G3BP2 (isoform b).

  • 经测试应用

    适用于: WB, IHC-P, Flow Cyt (Intra), Dot blotmore details
    不适用于: ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HeLa transfected with siRNA specifically targeting G3BP2, 293T, NIH/3T3, PC-12, Mouse brain, Mouse liver, Mouse testis, Rat brain, Rat liver, Rat testis, Human cerebellum, Mouse cerebral cortex, Mouse brain, Rat cerebellum and Rat cerebral cortex lysates. IHC-P: Human cerebrum, Mouse cerebrum and Rat cerebrum tissues. Flow Cyt (Intra): HeLa cell. Dot: His-tagged human G3BP2 recombinant protein.

  • 常规说明

    ab317688 is the carrier-free version of ab317687.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.2
    Constituent: 100% PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR27325-43

  • 同种型

    IgG

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317688于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF or IP.

靶标

  • 功能

    Probable scaffold protein that may be involved in mRNA transport.

  • 序列相似性

    Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.

  • 翻译后修饰

    Arg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • G3BP-2 antibody
      • G3BP2 antibody
      • G3BP2_HUMAN antibody
      • GAP SH3 domain-binding protein 2 antibody
      • Ras GTPase-activating protein-binding protein 2 antibody
      see all

    图片

    • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      All lanes : Anti-G3BP2 (isoform a) antibody [EPR27325-43] (ab317687) at 1/1000 dilution

      Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : HeLa transfected with siRNA specifically targeting G3BP2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

    • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      All lanes : Anti-G3BP2 (isoform a) antibody [EPR27325-43] (ab317687) at 1/1000 dilution

      Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    • Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Western blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      All lanes : Anti-G3BP2 (isoform a) antibody [EPR27325-43] (ab317687) at 1/1000 dilution

      Lanes 1 & 9 : Mouse brain tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Mouse testis tissue lysate
      Lane 4 : Rat brain tissue lysate
      Lane 5 : Rat liver tissue lysate
      Lane 6 : Rat testis tissue lysate
      Lane 7 : Human cerebellum tissue lysate
      Lane 8 : Mouse cerebral cortex tissue lysate
      Lane 10 : Rat cerebellum tissue lysate
      Lane 11 : Rat cerebral cortex tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 40,70 kDa why is the actual band size different from the predicted?



      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

      The bands beneath the target band (40 kDa) are likely to be degraded target fragments.

      Exposure time: Lanes 1-6: 10 seconds, Lanes 7-11: 48 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling G3BP2 (isoform a) with ab317687 at 1/100 (5.27 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Positive staining on neurons of human cerebrum (PMID: 33203845). The section was incubated with ab317687 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling G3BP2 (isoform a) with ab317687 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Positive staining on neurons of mouse cerebrum. The section was incubated with ab317687 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling G3BP2 (isoform a) with ab317687 at 1/2000 (0.264 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Positive staining on neurons of rat cerebrum. The section was incubated with ab317687 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Flow Cytometry (Intracellular) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Flow Cytometry (Intracellular) - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling G3BP2 (isoform a) with ab317687 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Dot Blot - Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

      This data was developed using ab317687, the same antibody clone in a different buffer formulation.

      Dot blot analysis of G3BP2 (isoform a) using ab317687 at 1:1000 (0.527 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

      Lane 1: His-tagged human G3BP2 recombinant protein
      Lane 2: His-tagged human G3BP1 recombinant protein

      Exposure time: 180 seconds.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody does not cross-react with human G3BP1.

      Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) as total protein control staining at 1/5000 dilution.

    • Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)
      Anti-G3BP2 (isoform a) antibody [EPR27325-43] - BSA and Azide free (ab317688)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317688？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317688 尚未被引用在任何文献中。

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