Anti-G-protein coupled receptor 30抗体(ab39742)

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
In order to understand the problems encountered more fully and be able to offer any tips which may improve the results observed so far, would you mind answering the following questions:
1. Sample preparation: Lysis buffer: Protease inhibitors: Reducing agent: Heated prior to loading on gel? Temperature/time:
2. Was a loading control used?
3. Which secondary antibody is being used? Has this been used with any other primary antibody before? What have been the results of this?
4. Order number (or if you do not have access to this the approximate delivery date and the delivery address used).
This information will also allow us to investigate this case internally and initiate additional testing where necessary.
If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.

Thank you for contacting us. Can you tell me how your oocyte samples were fixed and embedded? Were you using fluorescent or colorimetric detection? Fluorescent signal can easily fade after storage for a few days, whereas colorimetric signal will be stable for months to years at room temperature, provided that the samples are properly fixed and embedded. How were you storing your samples? Were they mounted to a slide and coverslipped, or were they in solution? The signal should be more stable when the samples are mounted. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us.

My Spanish colleague is currently out of the office.

About the ER alpha R andER beta R antibodies:
The antibodies Ariana suggested to you are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

About the anti-Mas (https://www.abcam.com/ab123326 and https://www.abcam.com/ab66030) and the anti-GPER R (https://www.abcam.com/ab39742):
To our knowledge,these antibodieshave not been tested in IP.
Therefore, I can offer a discount off a future purchase if you buythese antibodiesnow, testthem inIP and submit feedback to us in the form of an Abreview.
It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.
The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know which antibody you would like tobuy and test in IP. I will then send a discount code. This code must be issued before purchasingthe antibodies you want to test,so please wait for my reply before ordering.

2. Purchasethe antibody you want to testeither by phone, fax, or online (https://www.abcam.com).

3. Test it/them in IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Gracias por contactarnos.



He llevado a cabo una búsqueda en nuestro catálogo de anticuerpos contra los receptores indicados:



-Para MAS, tenemos dos opciones, pero lamentablemente ninguna de ellas se ha validado mediante IP, pero si en WB. Si queréis echar un vistazo a sus datasheets adjunto el enlace a ellas:



https://www.abcam.com/MAS1-antibody-ab123326.html



https://www.abcam.com/MAS1-antibody-ab66030.html



-Contra GPER, también hay dos anticuerpos disponibles, aunque me temo que tampoco se han usado en IP. Uno de ellos sin embargo se ha validado en WB:



https://www.abcam.com/G-protein-coupled-receptor-30-antibody-ab39742.html



-Contra ER alpha Receptor, hay varios anticuerpos validados mediante IP con células humanas, podéis echar un vistazo a las distintas alternativas en el siguiente enlace:



https://www.abcam.com/index.html?pageconfig=searchresults&search=ER%20alpha%20Receptor&pt=1&sk=app&sv=70&sn=IP&l=3&fViewMore=1



-Por último, para Estrogen Receptor beta, también hay dos alternativas validadas en IP con células humanas:



https://www.abcam.com/Estrogen-Receptor-beta-antibody-988-ab16813.html



https://www.abcam.com/Estrogen-Receptor-beta-antibody-EPR3777-ab92306.html






Siento que para los dos primeros receptores no haya una alternativa adecuada, sin embargo si queréis, podéis participar en nuestro sistema de evaluación de anticuerpos Abreview.

En Abcam ofrecemos un servicio de evaluación de anticuerpos, llamado Abreview, por el cual podemos ofrecerte un descuento (por valor de un anticuerpo primario / proteína) para una futura compra si adquieres el producto y lo testas en una aplicación o en una especie no testada hasta ahora (como IP), y publicas los resultados en nuestra website, en forma de Abreview, independientemente de que éstos sean positivos o negativos.

Si estás interesado en la oferta, házmelo saber antes de comprar los anticuerpos, para que te envie el código canjeable por un anticuerpo gratuito en la futura compra.

Para conocer con más detalle nuestro sistema de Abreview, visita la página: https://www.abcam.com/abreview.

No dudes en ponerte en contacto con nosotros si tienes cualquier otra consulta sobre esta oferta y estaré encantada de poder ayudarte. Si necesitas un presupuesto con alguno de los productos mencionados, también estaré encantada de enviártelo.