Anti-G-CSF抗体(ab9691)
Key features and details
- Rabbit polyclonal to G-CSF
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
-
产品名称
Anti-G-CSF抗体
参阅全部 G-CSF 一抗 -
描述
兔多克隆抗体to G-CSF -
宿主
Rabbit -
经测试应用
适用于: WB, Sandwich ELISAmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human G-CSF aa 1 to the C-terminus. Produced in E.coli. Isoform Short of G-CSF.
Database link: P09919-2 -
常规说明
Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab216607.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Lyophilized:Reconstitute with 200µl of sterile water. -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF |
靶标
-
功能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
序列相似性
Belongs to the IL-6 superfamily. -
翻译后修饰
O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
-
别名
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
图片
-
Western blot - Anti-G-CSF antibody (ab9691)
-
Sandwich ELISA - Anti-G-CSF antibody (ab9691)
数据表及文件
-
SDS download
-
Datasheet download
文献 (13)
ab9691 被引用在 13 文献中.
- Ray AL et al. G-CSF Is a Novel Mediator of T-Cell Suppression and an Immunotherapeutic Target for Women with Colon Cancer. Clin Cancer Res 29:2158-2169 (2023). PubMed: 36951682
- Kemuriyama K et al. Squamous cell carcinoma-derived G-CSF promotes tumor growth and metastasis in mice through neutrophil recruitment and tumor cell proliferation, associated with poor prognosis of the patients. Genes Cells 28:573-584 (2023). PubMed: 37248626
- Gunyeli I et al. Methotrexate-induced toxic effects and the ameliorating effects of astaxanthin on genitourinary tissues in a female rat model. Arch Gynecol Obstet N/A:N/A (2021). PubMed: 33608803
- Aslankoc R et al. Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats. J Biochem Mol Toxicol 34:e22445 (2020). PubMed: 31975554
- Liu L et al. Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling. J Mol Cell Biol 12:723-737 (2020). PubMed: 32242230