Anti-FZD3抗体(ab75233)
Key features and details
- Rabbit polyclonal to FZD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FZD3抗体
参阅全部 FZD3 一抗 -
描述
兔多克隆抗体to FZD3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide derived from an internal region of human FZD3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75233于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 76 kDa.
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| ICC/IF |
1/500 - 1/1000.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 76 kDa. |
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ICC/IF
1/500 - 1/1000. |
靶标
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功能
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. -
组织特异性
Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis. -
序列相似性
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
结构域
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
细胞定位
Membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7976 Human
- Omim: 606143 Human
- SwissProt: Q9NPG1 Human
- Unigene: 40735 Human
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别名
- Frizzled 3 antibody
- Frizzled 3 seven transmembrane spanning receptor antibody
- Frizzled family receptor 3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-FZD3 antibody (ab75233)ab75233 at 1/500 dilution staining FZD3 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Western blot - Anti-FZD3 antibody (ab75233)All lanes : Anti-FZD3 antibody (ab75233) at 1/500 dilution
Lane 1 : HUVEC cell extracts
Lane 2 : HUVEC cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 76 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab75233 被引用在 5 文献中.
- Ting W et al. Overexpression of microRNA-203 Suppresses Proliferation, Invasion, and Migration while Accelerating Apoptosis of CSCC Cell Line SCL-1. Mol Ther Nucleic Acids 21:428-440 (2020). PubMed: 32668390
- Zhao Y et al. NEAT1 regulates microtubule stabilization via FZD3/GSK3ß/P-tau pathway in SH-SY5Y cells and APP/PS1 mice. Aging (Albany NY) 12:23233-23250 (2020). PubMed: 33221742
- Mo D et al. A tRNA fragment, 5'-tiRNAVal, suppresses the Wnt/ß-catenin signaling pathway by targeting FZD3 in breast cancer. Cancer Lett 457:60-73 (2019). PubMed: 31078732
- Gammons MV et al. Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. J Cell Sci 129:3892-3902 (2016). Human . PubMed: 27744318
- Wang SB et al. Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle. Genet Mol Res 15:N/A (2016). PubMed: 26909900