Anti-Fyn抗体[FYN-01] (ab1881)
![Western blot - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.cn/ps/products/1/ab1881/Images/Fyn-Primary-antibodies-ab1881-2.jpg "Western blot - Anti-Fyn antibody [FYN-01] (ab1881)")
Key features and details
- Mouse monoclonal [FYN-01] to Fyn
- Suitable for: ICC/IF, IP, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Fyn抗体[FYN-01]
参阅全部 Fyn 一抗 -
描述
小鼠单克隆抗体[FYN-01] to Fyn -
宿主
Mouse -
特异性
The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases. -
经测试应用
适用于: ICC/IF, IP, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
FYN-01 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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IP
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa. |
靶标
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功能
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. -
组织特异性
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
细胞定位
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. - Information by UniProt
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数据库链接
- Entrez Gene: 2534 Human
- Entrez Gene: 14360 Mouse
- Omim: 137025 Human
- SwissProt: P06241 Human
- SwissProt: P39688 Mouse
- Unigene: 390567 Human
- Unigene: 4848 Mouse
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形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
图片
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Western blot - Anti-Fyn antibody [FYN-01] (ab1881)Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/ml +
Recombinant human Fyn protein (ab84696) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Exposure time: 10 seconds -
![Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.cn/ps/products/1/ab1881/Images/ab1881-455393-AntiFyn-antibody-Immunocytochemsitry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using ab1881 (red) at 5 µL. Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
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![Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.cn/ps/products/1/ab1881/Images/Fyn-Primary-antibodies-ab1881-3.jpg)
Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.cn/ps/products/1/ab1881/Images/ab1881-265226-anti-fyn-antibody-fyn-01-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 µL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue). -
![Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.cn/ps/products/1/ab1881/Images/ab1881-455392-AntiFyn-antibody-Immunocytochemsitry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)Immunocytochemistry analysis of Fyn in human HeLa cell line using ab1881 (green) at 5 µL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab1881 被引用在 11 文献中.
- Gray J et al. Heat shock protein amplification improves cerebellar myelination in the Npc1nih mouse model. EBioMedicine 86:104374 (2022). PubMed: 36455410
- Yu L et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). PubMed: 28791377
- Bouchet J et al. Rab11-FIP3 Regulation of Lck Endosomal Traffic Controls TCR Signal Transduction. J Immunol 198:2967-2978 (2017). PubMed: 28235866
- Suo M et al. Role of Fyn-mediated NMDA receptor function in prediabetic neuropathy in mice. J Neurophysiol 116:448-55 (2016). PubMed: 27146985
- Higa-Nakamine S et al. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem 290:25974-85 (2015). PubMed: 26338704