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Anti-Fyn抗体[2A10] (ab119855)

Submit a review Q&A (1)References (2)
Western blot - Anti-Fyn antibody [2A10] (ab119855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [2A10] (ab119855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [2A10] (ab119855)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [2A10] (ab119855)
  • Flow Cytometry - Anti-Fyn antibody [2A10] (ab119855)
  • ELISA - Anti-Fyn antibody [2A10] (ab119855)

Key features and details

  • Mouse monoclonal [2A10] to Fyn
  • Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Fyn抗体[2A10]
    参阅全部 Fyn 一抗

  • 描述

    小鼠单克隆抗体[2A10] to Fyn

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Human, Recombinant fragment

  • 免疫原

    Purified recombinant fragment of Human Fyn, expressed in E. coli.

  • 阳性对照

    • IHC-P: Human breast cancer and brain tissues ICC/IF: U251 cells Flow Cyt: Hela cells

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab119855于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/2000. Predicted molecular weight: 61 kDa.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/1000.
说明
WB
1/500 - 1/2000. Predicted molecular weight: 61 kDa.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/1000.

靶标

  • 功能

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.

  • 组织特异性

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 细胞定位

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt

  • 数据库链接

    • 形式

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

    • 别名

      • C syn protooncogene antibody
      • Fyn antibody
      • FYN oncogene related to SRC FGR YES antibody
      • FYN_HUMAN antibody
      • OKT3 induced calcium influx regulator antibody
      • P59 FYN antibody
      • p59-Fyn antibody
      • Protein tyrosine kinase fyn antibody
      • Proto oncogene tyrosine protein kinase fyn antibody
      • Proto-oncogene c-Fyn antibody
      • Proto-oncogene Syn antibody
      • Protooncogene Syn antibody
      • SLK antibody
      • Src like kinase antibody
      • Src yes related novel gene antibody
      • Src-like kinase antibody
      • Src/yes related novel antibody
      • SYN antibody
      • Tyrosine kinase p59fyn T antibody
      • Tyrosine kinase p59fyn(T) antibody
      • Tyrosine-protein kinase Fyn antibody
      see all

    图片

    • Western blot - Anti-Fyn antibody [2A10] (ab119855)
      Western blot - Anti-Fyn antibody [2A10] (ab119855)
      Anti-Fyn antibody [2A10] (ab119855) at 1/500 dilution + Fyn recombinant protein (7-176 amino acids)

      Predicted band size: 61 kDa



      Predicted MW of Recombinant Fragment : 44.3 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [2A10] (ab119855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [2A10] (ab119855)
      ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human breast cancer tissue by Immunohistochemistry, with DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [2A10] (ab119855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fyn antibody [2A10] (ab119855)
      ab119855, at a 1/200 dilution, staining Fyn in paraffin embedded Human brain tissue by Immunohistochemistry, with DAB staining.
    • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [2A10] (ab119855)
      Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [2A10] (ab119855)
      Immunofluorescence analysis of U251 cells, using ab119855 at a 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    • Flow Cytometry - Anti-Fyn antibody [2A10] (ab119855)
      Flow Cytometry - Anti-Fyn antibody [2A10] (ab119855)
      Flow Cytometric analysis of Hela cells, using ab119855 at a 1/200 dilution (green) and negative control (purple).
    • ELISA - Anti-Fyn antibody [2A10] (ab119855)
      ELISA - Anti-Fyn antibody [2A10] (ab119855)
      Elisa results for ab119855

    文献 (2)

    发表研究结果有使用 ab119855？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119855 被引用在 2 文献中.

    • Peng S  et al. FYN/TOPK/HSPB1 axis facilitates the proliferation and metastasis of gastric cancer. J Exp Clin Cancer Res 42:80 (2023). PubMed: 37016377
    • Yamada Y  et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). PubMed: 24626634

    客户评价及客户问答

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    提交评价 提交问题

    Question

    Dear technical team,
    Our customer would like to know immunogen information below antibodies.
    CCK4
    1. Ab104839, recombinant fragment
    RON
    1. Ab93129, whole protein?
    ROR2
    1. Ab92379, synthetic peptide
    2. Ab80174, Recombinant human protein
    Fgr
    1. In your homepage, it says that Ab81599 (anti-Fgr) is made by immunized with recombinant fragment of human Fgr. May I know the exact amino acid region which is covered by recombinant fragment?
    2. In your home page, it says that ab92302 (anti-Fgr) is made by synthetic peptide. May I know the exact amino acid region which is covered by peptide?
    3. Ab65007, synthetic peptide
    Fer
    1. 109740, truncated recombinant protein
    Brk
    1. Ab52273, truncated recombinant protein
    Fes
    1. Ab108418, synthetic peptide
    Frk
    Ab91623, recombinant fragment
    Fyn
    1. Ab119855, recombinant fragment
    Csk
    Ab125005, synthetic peptide
    Lyn
    Ab52563, truncated recombinant protein
    Check this please.

    Read More
    Answer

    Thank you for contacting us.
    CCK4
    ab104839 was generated against amino acids x-y.

    RON
    ab93129 was generated against a partial recombinant protein containing the protein sequence spanning 163-514. A his-tag was used in the protein.

    ROR2
    ab92379 The peptide maps between amino acids 40 and 65 of human ROR2 (UniProt: Q01974), i.e. an extracellular epitope is detected.
    ab80174 The antigen is recorded in our system as a his-tag fusion protein, without specifying whether it is a partial or full-length recombinant protein. We apologise that more information is currently not available.

    Fgr
    ab81599 was generated against amino acids 2-100.
    ab92302 The peptide maps between amino acids 25 and 50 of human FGR (UniProt: P09769)
    ab65007 The amino acid range for this immunogen is 61-110 from human FGR (P09769).

    Fer
    ab109740 The recombinant protein used as the immunogen for this antibody encodes amino acids x-y.
    Brk
    ab52273 was generated against amino acids 2-175.

    Fes
    ab108418 The peptide maps between amino acids 760 and 790 of human FES (UniProt: P07332).

    Frk
    ab91623 was generated against amino acids x-y.

    Fyn
    ab119855 was generated against amino acids 7-176

    Csk
    ab125005 The peptide maps between amino acids 75 and 105 of human CSK (UniProt: P41240)

    Lyn
    ab52563 see attachment

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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