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重组RabMAb

重组Anti-FYB抗体[EPR2547Y] (ab76006)

Reviews (1) Submit a question References (2)
Western blot - Anti-FYB antibody [EPR2547Y] (ab76006)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EPR2547Y] (ab76006)
  • Anti-FYB antibody [EPR2547Y] (ab76006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2547Y] to FYB
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-FYB抗体[EPR2547Y]
    参阅全部 FYB 一抗

  • 描述

    兔单克隆抗体[EPR2547Y] to FYB

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide within Human FYB aa 700-800 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • TF-1 lysate. Human tonsil tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76006于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
IHC-P
说明
应用说明
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/500. Predicted molecular weight: 85 kDa.

Is unsuitable for Flow Cyt or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 功能

    Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.

  • 组织特异性

    Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.

  • 序列相似性

    Contains 1 SH3 domain.

  • 翻译后修饰

    T-cell receptor ligation leads to increased tyrosine phosphorylation.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ADAP antibody
      • Adhesion and degranulation promoting adaptor protein antibody
      • FYB 120/130 antibody
      • Fyb antibody
      • FYB-120/130 antibody
      • FYB_HUMAN antibody
      • FYN binding protein antibody
      • FYN T binding protein antibody
      • FYN-binding protein antibody
      • FYN-T-binding protein antibody
      • p120/p130 antibody
      • PRO0823 antibody
      • SLAP 130 antibody
      • SLAP-130 antibody
      • SLAP130 antibody
      • SLP 76 associated phosphoprotein antibody
      • SLP-76-associated phosphoprotein antibody
      • SLP76 associated phosphoprotein antibody
      see all

    图片

    • Western blot - Anti-FYB antibody [EPR2547Y] (ab76006)
      Western blot - Anti-FYB antibody [EPR2547Y] (ab76006)
      Anti-FYB antibody [EPR2547Y] (ab76006) at 1/500 dilution + TF-1 lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 85 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EPR2547Y] (ab76006)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EPR2547Y] (ab76006)

      ab76006, at 1/100 dilution, staining FYB in human tonsil by immunohistochemistry using paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-FYB antibody [EPR2547Y] (ab76006)
      Anti-FYB antibody [EPR2547Y] (ab76006)

    文献 (2)

    发表研究结果有使用 ab76006？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76006 被引用在 2 文献中.

    • Wu Z  et al. Fyn-binding protein ADAP supports actin organization in podocytes. Physiol Rep 5:N/A (2017). WB ; Mouse . PubMed: 29192064
    • Thiere M  et al. Integrin Activation Through the Hematopoietic Adapter Molecule ADAP Regulates Dendritic Development of Hippocampal Neurons. Front Mol Neurosci 9:91 (2016). PubMed: 27746719

    客户评价及客户问答

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    Western blot abreview for Anti-FYB antibody [EPR2547Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (CHO cell transfected)
    Loading amount
    20 µg
    Specification
    CHO cell transfected
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 20 2012

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