Anti-FUZ抗体(ab122742)
Key features and details
- Rabbit polyclonal to FUZ
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FUZ抗体
参阅全部 FUZ 一抗 -
描述
兔多克隆抗体to FUZ -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: LLRNFYTLVT STHFPPEPGP PEKTEDEVYQ AQLPRACYLV LGTEEPGTGV RLVALQLGLR RLLLLLSPQS PTHGLRSLAT HTLHALTP, corresponding to amino acids 329-416 of Human FUZ.
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阳性对照
- Human smooth muscle tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122742于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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功能
Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. May regulate the morphogenesis of hair follicles which depends on functional primary cilia. -
序列相似性
Belongs to the fuzzy family. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 80199 Human
- Omim: 610622 Human
- SwissProt: Q9BT04 Human
- Unigene: 288800 Human
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别名
- FLJ22688 antibody
- fuz antibody
- FUZZY_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-FUZ antibody (ab122742)Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUZ antibody (ab122742)ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry. -
Western blot - Anti-FUZ antibody (ab122742)Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122742 被引用在 1 文献中.
- He M et al. In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer. Life Sci 197:91-100 (2018). PubMed: 29421438