Anti-FUT3抗体(ab110082)
Key features and details
- Rabbit polyclonal to FUT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FUT3抗体
参阅全部 FUT3 一抗 -
描述
兔多克隆抗体to FUT3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A synthesized peptide derived from an internal sequence of FUT3.
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阳性对照
- Human colon carcinoma tissue; extracts from K562 cells and Jurkat cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110082于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 42 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 42 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. -
组织特异性
Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 10 family. -
细胞定位
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi. - Information by UniProt
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数据库链接
- Entrez Gene: 2525 Human
- Omim: 111100 Human
- SwissProt: P21217 Human
- Unigene: 169238 Human
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别名
- Alpha-(1,3/1,4)-fucosyltransferase antibody
- Blood group Lewis alpha-4-fucosyltransferase antibody
- CD174 antibody
see all
图片
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Western blot - Anti-FUT3 antibody (ab110082)All lanes : Anti-FUT3 antibody (ab110082) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT3 antibody (ab110082)Immunohistochemical analysis of Blood Group Lewis b in paraffin embedded Human colon carcinoma tissue, using a 1/50 dilution of ab110082 with (right image) or without (left image) immunizig peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab110082 被引用在 3 文献中.
- Esposito M et al. Bone vascular niche E-selectin induces mesenchymal-epithelial transition and Wnt activation in cancer cells to promote bone metastasis. Nat Cell Biol N/A:N/A (2019). PubMed: 30988423
- Zhan L et al. Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF-ß induced EMT in pancreatic cancer cells. Oncol Lett 16:924-930 (2018). PubMed: 29963165
- Meng L et al. High expression of FUT3 is linked to poor prognosis in clear cell renal cell carcinoma. Oncotarget 8:61036-61047 (2017). PubMed: 28977844