Anti-Furin抗体(ab3467)
Key features and details
- Rabbit polyclonal to Furin
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Furin抗体
参阅全部 Furin 一抗 -
描述
兔多克隆抗体to Furin -
宿主
Rabbit -
特异性
Detects Furin from canine and mouse cells as well as transfected human Furin. This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Furin aa 780-793.
Sequence:RGERTAFIKDQSAL
(Peptide available asab4989) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 2 µg/ml.
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| WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).Can be blocked with Furin peptide (ab4989).
Representing Furin in BSC-40 cells transfected with the human Furin gene. |
| 说明 |
|---|
|
ICC
Use a concentration of 2 µg/ml. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).Can be blocked with Furin peptide (ab4989). Representing Furin in BSC-40 cells transfected with the human Furin gene. |
靶标
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功能
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
组织特异性
Seems to be expressed ubiquitously. -
序列相似性
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
结构域
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
翻译后修饰
The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
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数据库链接
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Omim: 136950 Human
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
- Unigene: 513153 Human
- Unigene: 5241 Mouse
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别名
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
图片
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Immunocytochemistry - Anti-Furin antibody (ab3467)
Immunocytochemical analysis of methanol-fixed 2% BSA blocked HeLa cells strining furin with ab3467 at 2 µg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and the cytoskeleton with alpha-Tubulin Monoclonal Antibody and Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate at 1/400 dilution (red). Negative control uses no primary antibody
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Western blot - Anti-Furin antibody (ab3467)All lanes : Anti-Furin antibody (ab3467) at 1/500 dilution
Lane 1 : HeLa lysate at 30 µg
Lane 2 : HEK-293 extract at 30 µg
Lane 3 : K-562 extract at 30 µg
Lane 4 : SH-SY5Y extract at 30 µg
Lane 5 : conditioned media from HeLa cell line at 10 µg
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa -
Immunocytochemistry - Anti-Furin antibody (ab3467)Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.
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Western blot - Anti-Furin antibody (ab3467)All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide. -
Immunocytochemistry - Anti-Furin antibody (ab3467)Image courtesy of an anonymous Abreview.ab3467 staining Furin in human A549 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA/ 5%FBS/PBS for 1 hour at 25°C and then incubated with ab3467 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.
实验方案
数据表及文件
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Datasheet download
文献 (24)
ab3467 被引用在 24 文献中.
- O'Sullivan MJ et al. In well-differentiated primary human bronchial epithelial cells, TGF-ß1 and TGF-ß2 induce expression of furin. Am J Physiol Lung Cell Mol Physiol 320:L246-L253 (2021). PubMed: 33174447
- Papa G et al. Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion. PLoS Pathog 17:e1009246 (2021). PubMed: 33493182
- Wang H et al. The role of high cholesterol in age-related COVID19 lethality. bioRxiv N/A:N/A (2021). PubMed: 32511366
- Akerman AW et al. miR-133a Replacement Attenuates Thoracic Aortic Aneurysm in Mice. J Am Heart Assoc 10:e019862 (2021). PubMed: 34387094
- Ahn JH et al. Nasal ciliated cells are primary targets for SARS-CoV-2 replication in the early stage of COVID-19. J Clin Invest 131:N/A (2021). PubMed: 34003804
