Anti-Furin抗体(ab28547)
Key features and details
- Rabbit polyclonal to Furin
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Furin抗体
参阅全部 Furin 一抗 -
描述
兔多克隆抗体to Furin -
宿主
Rabbit -
特异性
ab28547 recognizes subtilisin-like serine protease furin. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Furin (N terminal).
(Peptide available asab41397) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody has been peptide-affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28547于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 88 kDa.
1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection. |
说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 88 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection. |
靶标
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功能
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
组织特异性
Seems to be expressed ubiquitously. -
序列相似性
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
结构域
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
翻译后修饰
The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
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数据库链接
- Entrez Gene: 18550 Mouse
- Entrez Gene: 54281 Rat
- SwissProt: P23188 Mouse
- SwissProt: P23377 Rat
- Unigene: 5241 Mouse
- Unigene: 3220 Rat
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别名
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab28547 被引用在 6 文献中.
- Braun E et al. Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins. Cell Rep 27:2092-2104.e10 (2019). PubMed: 31091448
- Jaaks P et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget 7:76743-76755 (2016). Human . PubMed: 27572312
- Jaaks P et al. The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion. PLoS One 11:e0161396 (2016). IHC . PubMed: 27548722
- Sathyamurthy M et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . PubMed: 25219685
- Cekic M et al. Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Restor Neurol Neurosci 30:115-26 (2012). WB ; Rat . PubMed: 22232032
- Hajdin K et al. Furin targeted drug delivery for treatment of rhabdomyosarcoma in a mouse model. PLoS One 5:e10445 (2010). IHC-Fr ; Human . PubMed: 20454619