AB28547

Anti-Furin抗体

Name: Furin抗体 (ab28547)| Abcam中文官网
Brand: Abcam Limited
SKU: AB28547
Price: 4903 CNY
Availability: InStock

Anti-Furin antibody

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(6 Publications)

Rabbit Polyclonal FURIN antibody. Suitable for WB and reacts with Recombinant fragment samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human FURIN.

查看别名

FUR, PACE, PCSK3, FURIN, Furin, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme

1 Images

Unknown

Western blot - Anti-Furin antibody (AB28547)

All lanes:

Western blot - Anti-Furin antibody (ab28547) at 1/1000 dilution

Lane 1:

Furin at 0.05 µg

Lane 2:

Furin at 0.01 µg

Lane 3:

Furin at 0.001 µg

Predicted band size: 87 kDa

Observed band size: 88 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

应用

applications

免疫原

Synthetic Peptide within Human FURIN. The exact immunogen used to generate this antibody is proprietary information.

P09958

特异性

ab28547 recognizes subtilisin-like serine protease furin.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

This antibody has been peptide-affinity purified.

存储溶液

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Furin is a serine endoprotease that plays critical role in the cleavage of precursor proteins into their active forms. It belongs to the proprotein convertase family and is also known as furin protease. The molecular weight of furin is approximately 54 kDa. Furin is ubiquitously expressed in various tissues in the body with high expression levels in the liver and the central nervous system. Furin is mainly located in the trans-Golgi network where it processes many precursor proteins involved in various cellular functions.

Biological function summary

Furin processes precursor proteins by cleaving at specific sequence motifs. This activity is essential for the maturation of many hormones growth factors and receptors. Furin is not part of a larger complex; it functions independently in its proteolytic activity. The processing by furin ensures that proteins become functional affecting numerous biological processes across different cell types and tissues.

Pathways

The activity of furin impacts the regulation of the Notch signaling pathway and the MAPK/ERK pathway. In the Notch signaling pathway furin processes Notch receptors facilitating their activation and downstream signaling. In the MAPK/ERK pathway furin activates several key proteins including growth factors and receptors essential for cell proliferation and differentiation. Furin-related proteins such as convertase enzymes are part of a similar family having roles in overlapping or complementary pathways.

Furin is implicated in several conditions including cardiovascular diseases and certain cancers. In cardiovascular diseases furin's role in processing proteins such as pro-BNP may have implications in cardiac function and disease progression. In cancer altered furin activity can lead to the inappropriate activation of growth factors and receptors contributing to tumor progression. Furin inhibitors are researched for their potential to modulate furin activity in these diseases and provide therapeutic benefits.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed : 11799113, PubMed : 1629222, PubMed : 1713771, PubMed : 2251280, PubMed : 24666235, PubMed : 25974265, PubMed : 7592877, PubMed : 7690548, PubMed : 9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed : 7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed : 20489134, PubMed : 21763278). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (By similarity).. (Microbial infection) Cleaves and activates diphtheria toxin DT.. (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA).. (Microbial infection) Cleaves and activates HIV-1 virus Envelope glycoprotein gp160.. (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin.. (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP.. (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells.. (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F.

See full target information FURIN

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 27:2092-2104.e10 PubMed31091448

2019

Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth Braun,Dominik Hotter,Lennart Koepke,Fabian Zech,Rüdiger Groß,Konstantin M J Sparrer,Janis A Müller,Christian K Pfaller,Elena Heusinger,Rebecka Wombacher,Kathrin Sutter,Ulf Dittmer,Michael Winkler,Graham Simmons,Martin R Jakobsen,Karl-Klaus Conzelmann,Stefan Pöhlmann,Jan Münch,Oliver T Fackler,Frank Kirchhoff,Daniel Sauter

Oncotarget 7:76743-76755 PubMed27572312

2016

The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells.

Applications

Unspecified application

Species

Human

Patricia Jaaks,Gianmarco Meier,Nagjie Alijaj,Eva Brack,Peter Bode,Ewa Koscielniak,Marco Wachtel,Beat W Schäfer,Michele Bernasconi

PloS one 11:e0161396 PubMed27548722

2016

The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion.

Applications

IHC

Species

Unspecified reactive species

Patricia Jaaks,Valentina D'Alessandro,Nicole Grob,Sina Büel,Katarina Hajdin,Beat W Schäfer,Michele Bernasconi

Biotechnology and bioengineering 112:560-8 PubMed25219685

2014

Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7.

Applications

Species

Unspecified reactive species

Madhavi Sathyamurthy,Che Lin Kim,You Lim Bang,Young Sik Kim,Ju Woong Jang,Gyun Min Lee

Restorative neurology and neuroscience 30:115-26 PubMed22232032

2012

Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury.

Applications

Species

Rat

Milos Cekic,Sarah J Johnson,Vinay H Bhatt,Donald G Stein

PloS one 5:e10445 PubMed20454619

2010

Furin targeted drug delivery for treatment of rhabdomyosarcoma in a mouse model.

Applications

IHC-Fr

Species

Human

Katarina Hajdin,Valentina D'Alessandro,Felix K Niggli,Beat W Schäfer,Michele Bernasconi

View all publications

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Anti-Furin抗体

Anti-Furin antibody

Western blot - Anti-Furin antibody (AB28547)