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Anti-Furin抗体(ab177231)

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Western blot - Anti-Furin antibody (ab177231)

    Key features and details

    • Goat polyclonal to Furin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Furin抗体
      参阅全部 Furin 一抗

    • 描述

      山羊多克隆抗体to Furin

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Cow

    • 免疫原

      Synthetic peptide corresponding to Human Furin aa 104-116 (internal sequence) (Cysteine residue).
      Sequence:

      RTKRDVYQEPTDP


      Database link: NP_002560.1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • HEK293 lysate overexpressing Human Furin with C-terminal MYC tag.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 99% Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      ab177231 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab177231于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa.

    A 1 hour primary incubation is recommended for this product.
    说明
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa.

    A 1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.

    • 组织特异性

      Seems to be expressed ubiquitously.

    • 序列相似性

      Belongs to the peptidase S8 family. Furin subfamily.
      Contains 1 homo B/P domain.

    • 结构域

      Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.

    • 翻译后修饰

      The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
      Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.

    • 细胞定位

      Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Dibasic processing enzyme antibody
      • Dibasic-processing enzyme antibody
      • FES upstream region antibody
      • FUR antibody
      • FURIN antibody
      • Furin membrane associated receptor protein antibody
      • FURIN_HUMAN antibody
      • PACE antibody
      • Paired basic amino acid residue cleaving enzyme antibody
      • Paired basic amino acid residue-cleaving enzyme antibody
      • PCSK3 antibody
      • Proprotein convertase subtilisin/kexin type 3 antibody
      • SPC1 antibody
      see all

    图片

    • Western blot - Anti-Furin antibody (ab177231)
      Western blot - Anti-Furin antibody (ab177231)
      Lanes 1-2 : Anti-Furin antibody (ab177231) at 1 µg/ml
      Lane 3 : anti-MYC tag at 1/1000 dilution

      Lanes 1 & 3 : HEK293 lysate (in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag
      Lane 2 : Mock-transfected HEK293 lysate (in RIPA buffer)

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 87 kDa



      Primary incubations were for 1 hour.

    文献 (0)

    发表研究结果有使用 ab177231？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab177231 尚未被引用在任何文献中。

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