Anti-FUNDC1抗体(ab224722)
Key features and details
- Rabbit polyclonal to FUNDC1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FUNDC1抗体
参阅全部 FUNDC1 一抗 -
描述
兔多克隆抗体to FUNDC1 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow
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免疫原
Recombinant fragment corresponding to Human FUNDC1 aa 1-100.
Database link: Q8IVP5 -
阳性对照
- WB: HeLa, MCF7, A549, U251, K562, Mouse and Rat brain, and Mouse and Rat liver lysates. IHC-P: Human stomach tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224722于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
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| IHC-P |
1/20 - 1/200.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
|
IHC-P
1/20 - 1/200. |
靶标
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功能
Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the FUN14 family. -
结构域
The YXXL motif mediates the interaction with MAP1 LC3 family proteins MAP1LC3A, MAP1LC3B and GABARAP (PubMed:22267086). -
翻译后修饰
Phosphorylation at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy. -
细胞定位
Mitochondrion outer membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 518258 Cow
- Entrez Gene: 139341 Human
- Entrez Gene: Mouse
- Entrez Gene: 72018 Mouse
- Entrez Gene: 363442 Rat
- Omim: 300871 Human
- SwissProt: F1N5S9 Cow
- SwissProt: Q8IVP5 Human
see all -
别名
- FUN14 domain containing 1 antibody
- FUN14 domain containing protein 1 antibody
- FUN14 domain-containing protein 1 antibody
see all
图片
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Western blot - Anti-FUNDC1 antibody (ab224722)All lanes : Anti-FUNDC1 antibody (ab224722) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : U251 whole cell lysate
Lane 5 : K562 whole cell lysate
Secondary
All lanes : Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Western blot - Anti-FUNDC1 antibody (ab224722)All lanes : Anti-FUNDC1 antibody (ab224722) at 1/1000 dilution
Lane 1 : Mouse Brain tissue lysate
Lane 2 : Mouse Liver tissue lysate
Lane 3 : Rat Brain tissue lysate
Lane 4 : Rat Liver tissue lysate
Secondary
All lanes : Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (ab224722)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling FUNDC1 with ab224722 at 1/50 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab224722 被引用在 22 文献中.
- Wang Q et al. NLRX1 increases human retinal pigment epithelial autophagy and reduces H2O2-induced oxidative stress and inflammation by suppressing FUNDC1 phosphorylation and NLRP3 activation. Allergol Immunopathol (Madr) 51:177-186 (2023). PubMed: 36617838
- Ma L et al. Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy. Redox Biol 62:102693 (2023). PubMed: 37030149
- Wu Y et al. Poricoic acid A induces mitophagy to ameliorate podocyte injury in diabetic kidney disease via downregulating FUNDC1. J Biochem Mol Toxicol 37:e23503 (2023). PubMed: 37706594
- Yu X et al. Jionoside A1 alleviates ischemic stroke ischemia/reperfusion injury by promoting Nix-mediated mitophagy. Cell Mol Biol (Noisy-le-grand) 69:237-245 (2023). PubMed: 37715375
- Ye H et al. Focused low-intensity pulsed ultrasound alleviates osteoarthritis via restoring impaired FUNDC1-mediated mitophagy. iScience 26:107772 (2023). PubMed: 37720103