重组Anti-FUBP3抗体[EPR13174-61] (ab181122)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13174-61] to FUBP3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FUBP3抗体[EPR13174-61]
参阅全部 FUBP3 一抗 -
描述
兔单克隆抗体[EPR13174-61] to FUBP3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- MCF7, U87-MG or HepG2 lysate, Human kidney clear cell carcinoma or colon tissue, HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13174-61 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181122于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
|
WB |
1/1000 - 1/20000. Detects a band of approximately 62 kDa.
|
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ICC/IF |
1/50 - 1/100.
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说明 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
WB
1/1000 - 1/20000. Detects a band of approximately 62 kDa. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. -
组织特异性
Detected in a number of cell lines. -
序列相似性
Contains 4 KH domains. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8939 Human
- Entrez Gene: 320267 Mouse
- Entrez Gene: 362106 Rat
- Omim: 603536 Human
- SwissProt: Q96I24 Human
- Unigene: 673029 Human
- Unigene: 98751 Human
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别名
- Far upstream element (FUSE) binding protein 3 antibody
- Far upstream element binding protein 3 antibody
- Far upstream element-binding protein 3 antibody
see all
图片
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Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/5000 dilution + HepG2 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution -
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling FUBP3 with ab181122 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling FUBP3 with ab181122 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
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All lanes : Anti-FUBP3 antibody [EPR13174-61] (ab181122) at 1/20000 dilution
Lane 1 : MCF7 lysate
Lane 2 : U87-MG lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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