Name: Anti-FUBP1/FBP antibody [EPR12327]
Brand: RabMAb
SKU: ab181111
Rating: 5 (2 reviews)

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12327] to FUBP1/FBP
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

产品名称

Anti-FUBP1/FBP抗体[EPR12327]
参阅全部 FUBP1/FBP 一抗
描述

兔单克隆抗体[EPR12327] to FUBP1/FBP
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Jurkat, HeLa, Raji and HepG2 whole cell lysate (ab7900), rat brain, mouse brain and mouse spleen tissue lysates. IHC-P: Human breast carcinoma and pancreas tissues. ICC/IF: HeLa and HepG2 cells. Flow Cyt (intra): Jurkat cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR12327
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab181111于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/60 - 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa). For unpurified use at 1/1000 - 1/20000.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF	(1)	1/250 - 1/500.

说明
Flow Cyt (Intra) 1/60 - 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa). For unpurified use at 1/1000 - 1/20000.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/250 - 1/500.

功能

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
序列相似性

Contains 4 KH domains.
翻译后修饰

Ubiquitinated. This targets the protein for proteasome-mediated degradation.
细胞定位

Nucleus.
Target information above from: UniProt accession Q96AE4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 8880 Human
- Entrez Gene: 51886 Mouse
- Entrez Gene: 654496 Rat
- Omim: 603444 Human
- SwissProt: Q96AE4 Human
- SwissProt: Q91WJ8 Mouse
- SwissProt: Q32PX7 Rat
- Unigene: 567380 Human
- Unigene: 278922 Mouse
- Unigene: 177078 Rat
see all
别名
- DNA helicase V antibody
- far upstream element (FUSE) binding protein 1 antibody
- Far upstream element (FUSE) binding protein 4 antibody
- Far upstream element binding protein 1 antibody
- far upstream element binding protein antibody
- Far upstream element-binding protein 1 antibody
- FBP antibody
- FUBP antibody
- Fubp1 antibody
- FUBP1_HUMAN antibody
- Fubp4 antibody
- FUSE binding protein 1 antibody
- FUSE-binding protein 1 antibody
- HDH V antibody
see all

Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/2000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FUBP1/FBP with purified ab181111 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FUBP1/FBP with purified ab181111 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Intracellular Flow Cytometry analysis of Jurkat cells labelling FUBP1 / FBP with purified ab181111 at 1/60 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (unpurified) + Jurkat cell lysate at 20 µg

Secondary
Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Western blot - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Immunocytochemistry/Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling FUBP1/FBP with unpurified ab181111 at 1/500 dilution (red) followed by Alexa Fluor^® 555-conjugated goat anti rabbit IgG secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow Cytometry (Intracellular) - Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/90 (pink), compared to a rabbit monoclonal IgG isotype control (green), followed by goat anti rabbit IgG (FITC) secondary antibody at a dilution of 1/150.
Anti-FUBP1/FBP antibody [EPR12327] (ab181111)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab181111？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab181111 被引用在 13 文献中.

Wang P et al. Icariin activates far upstream element binding protein 1 to regulate hypoxia-inducible factor-1α and hypoxia-inducible factor-2α signaling and benefits chondrocytes. PeerJ 11:e15917 (2023). PubMed: 37637163
Olguin SL et al. KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization. Commun Biol 5:672 (2022). PubMed: 35798971
Yang B et al. TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. J Immunol Res 2021:9994004 (2021). PubMed: 33987449
Wang H et al. Protein and Signaling Pathway Responses to rhIL-6 Intervention Before Lobaplatin Treatment in Osteosarcoma Cells. Front Oncol 11:602712 (2021). PubMed: 33791202
Wen H et al. Long non-coding RNA KRT8P41/miR-193a-3p/FUBP1 axis modulates the proliferation and invasion of chordoma cells. J Bone Oncol 31:100392 (2021). PubMed: 34712553

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Brain neural stem cell)

Permeabilization

Yes - 0.2% Triton X 100

Specification

Brain neural stem cell

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 22 2015

Application

Immunoprecipitation

Sample

Mouse Cell lysate - whole cell (Brain neural stem cell)

Total protein in input

300 µg

Immuno-precipitation step

Protein G

Specification

Brain neural stem cell

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 22 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (13)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-FUBP1/FBP antibody [EPR12327]

Immunoprecipitation abreview for Anti-FUBP1 / FBP antibody [EPR12327]