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AB259560

重组Anti-FSTL3抗体[EPR21996-225] - BSA and Azide free (Detector)

Anti-FSTL3 antibody [EPR21996-225] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal FSTL3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

FLRG, UNQ674/PRO1308, FSTL3, Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein

1 Images
Sandwich ELISA - Anti-FSTL3 antibody [EPR21996-225] - BSA and Azide free (Detector) (AB259560)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FSTL3 antibody [EPR21996-225] - BSA and Azide free (Detector) (AB259560)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab245715).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21996-225

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab259560 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human FLRG. The recommended pair for sandwich ELISA is:
Capture: ab259559, Human FLRG Capture Antibody (unconjugated)
Detector: ab259560, Human FLRG Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein FSTL3 also known as Follistatin-like 3 has a molecular mass of approximately 26 kDa. Mechanically FSTL3 acts by binding and neutralizing specific members of the TGF-beta superfamily influencing cellular processes like growth differentiation and apoptosis. It is mostly expressed in tissues such as the placenta pancreas and muscle where it plays a role in various developmental and physiological events.
Biological function summary

FSTL3 serves an important function in regulating cellular dynamics by modulating activity of ligands in the TGF-beta signaling pathway. It forms a complex with activins and other growth factors to inhibit their signaling capabilities. This interaction is key in processes like managing fetal development ensuring proper organogenesis and maintaining metabolic activities.

Pathways

Extensive research places FSTL3 within the TGF-beta signaling pathway and the insulin-like growth factor (IGF) system. These pathways are critical for managing cellular growth and metabolism. FSTL3 interacts with proteins such as activins and inhibins which share roles in regulating cell proliferation and differentiation linking FSTL3 directly to these essential cellular pathways.

FSTL3 links to pathologies like type 2 diabetes and muscle wasting. FSTL3 influences insulin signaling therefore its dysregulation can contribute to insulin resistance a hallmark of type 2 diabetes. It also associates with muscle protein wasting through its interaction with activin suggesting a potential therapeutic target in conditions like cachexia.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such as activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.
See full target information FSTL3
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搭配使用产品

Assay Kits

AB245715

Human FLRG ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FLRG ELISA Kit (AB245715)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB181141

Anti-IGFBP1 antibody [EPR14472(B)]

RabMAb|Recombinant

Western blot - Anti-IGFBP1 antibody [EPR14472(B)] (AB181141)

  • 3
  • 1
Primary Antibodies

AB174843

Anti-NBL1 antibody [EPR12397]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NBL1 antibody [EPR12397] (AB174843)

  • 0
  • 0
Primary Antibodies

AB219560

Anti-IGFBP6 antibody [EPR21809]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IGFBP6 antibody [EPR21809] (AB219560)

  • 0
  • 0

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