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Signal Transduction Growth Factors/Hormones Hormones

Anti-FSH抗体[INN-hFSH-117] (ab21011)

  • Datasheet
  • SDS
Submit a review Q&A (2)

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Key features and details

  • Mouse monoclonal [INN-hFSH-117] to FSH
  • Suitable for: ELISA, RIA
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-FSH抗体[INN-hFSH-117]
  • 描述

    小鼠单克隆抗体[INN-hFSH-117] to FSH
  • 宿主

    Mouse
  • 特异性

    Specific for Human Follicle Stimulating Hormone (FSH) intact. No cross-reaction is detected in RIA with human FSH beta or any other human glycoprotein hormone or subunits thereof.
  • 经测试应用

    适用于: ELISA, RIAmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Human FSH

  • 表位

    ab21011 recognizes the c1 epitope, a conformational epitope which is only detectable when the alpha and beta subunits of FSH are associated (intact FSH).
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    >95% pure (SDS-PAGE).
  • 克隆

    单克隆
  • 克隆编号

    INN-hFSH-117
  • 骨髓瘤

    x63-Ag8.653
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Developmental Biology
    • Reproduction
    • Hormones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab21011于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.

靶标

  • 相关性

    Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one (see also luteinizing hormone, chorionic gonadotropin, and thyroid stimulating hormone), consist of a shared alpha chain and a beta chain encoded by a separate gene. The FSHB gene encodes the beta subunit of follicle stimulating hormone.
  • 细胞定位

    Secreted
  • 数据库链接

    • Entrez Gene: 1081 Human
    • Entrez Gene: 2488 Human
    • Omim: 118850 Human
    • Omim: 136530 Human
    • SwissProt: P01215 Human
    • SwissProt: P01225 Human
    • 别名

      • Anterior pituitary glycoprotein hormones common subunit alpha antibody
      • CG alpha antibody
      • CGA antibody
      • Choriogonadotropin alpha chain antibody
      • Chorionic gonadotrophin alpha subunit antibody
      • Chorionic gonadotropin alpha polypeptide antibody
      • Follicle stimulating hormone alpha chain antibody
      • Follicle Stimulating Hormone Beta antibody
      • Follicle stimulating hormone beta polypeptide antibody
      • Follicle stimulating hormone beta subunit antibody
      • Follitropin alpha chain antibody
      • Follitropin beta chain antibody
      • Follitropin subunit beta antibody
      • FSH A antibody
      • FSH alpha antibody
      • FSH B antibody
      • FSH beta antibody
      • FSHA antibody
      • FSHB antibody
      • Glycoprotein hormones alpha chain antibody
      • Glycoprotein hormones alpha polypeptide antibody
      • GPHA 1 antibody
      • GPHa antibody
      • GPHA1 antibody
      • HCG antibody
      • LHA antibody
      • LSH alpha antibody
      • Luteinizing hormone alpha chain antibody
      • Lutropin alpha chain antibody
      • OTTHUMP00000016825 antibody
      • Thyroid stimulating hormone alpha chain antibody
      • Thyrotropin alpha chain antibody
      • TSH alpha antibody
      • TSHA antibody
      see all

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab21011?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab21011 尚未被引用在任何文献中。

    客户评价及客户问答

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    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    sELISA in mouse and rat for TSH, FSH and Luteinizing Hormone
    pairs:
    Luteinizing Hormone: ab72837 (mouse), ab72838 (mouse) - test in mouse, rat
    FSH: ab21011 (mouse, capture), ab30543 (HRP)- test in sELISA, mouse, rat
    TSH: ab6070 (HRP), ab6069 - test in mouse, rat
    send info on proteins for standard curve and secondary Abs (anti-mouse IgG) if required
    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP

    Read More

    Abcam community

    Verified customer

    Asked on Mar 08 2012

    Answer

    Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA

    Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

    In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

    The suggested secondary antibodies are:
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

    Testing Discount Codes:

    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in sELISA, mouse, rat
    DISCOUNT CODE: xxx-test in sELISA, mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat

    DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
    DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    回复于 Mar 08 2012

    Question

    Blockiert dieser AK die Bindestelle von FSH an Rezeptor?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2011

    Answer

    Vielen Dank für Ihren Anruf. Bitte entschuldigen Sie, dass meine Antwort so lange auf sich warten ließ. Das Labor, von dem wir den FSH Antikörperklon [INN-hFSH-117] beziehen, hat mir bestätigt, dass der Antikörper das intakte FSH am c1-Epitop bindet, welches durch die alpha- und beta-Untereinheit gebildet wird. Dieses Epitop ist den alpha 1, alpha 2 und alpha 3-Epitopen benachbart; detaillierte Informationen waren leider nicht zu erfahren. Meinen Recherchen zufolge wurde der Klon in der Publikation von Berger et al (PMID 2458911) charakterisiert, die füruns leider nicht zugänglich ist, von der ich aber hoffe, dass sie relevante Informationen für Sie enthält. Unseres Wissens nach wurde der Antikörper ab21011 bisher noch nicht auf seine etwaige Blockierung der Rezeptorbindestelle (d.h. in Rezeptorbindungsstudien/Inhibition assays) getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden. Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten: 1.) Bestätigen Sie mir bitte, dass Sie ab21011 in Rezeptorbindungsstudien testen möchten. 2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes. 3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax 4.) Testen Sie den Antikörper in Rezeptorbindungsstudien. 5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes" Unter der folgenden URL können Sie mehr über unser Abreview System erfahren: https://www.abcam.com/abreviews 6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss. Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Abcam Scientific Support

    回复于 Dec 06 2011

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