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Anti-FSBA抗体[2F5/5] (ab18575)

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Key features and details

  • Mouse monoclonal [2F5/5] to FSBA
  • Suitable for: ELISA, IP
  • Reacts with: Species independent
  • Isotype: IgG

概述

  • 产品名称

    Anti-FSBA抗体[2F5/5]

  • 描述

    小鼠单克隆抗体[2F5/5] to FSBA

  • 宿主

    Mouse

  • 经测试应用

    适用于: ELISA, IPmore details

  • 种属反应性

    与反应: Species independent

  • 常规说明

    FSBA 2F5/5 has been shown to detect Protein Kinase C-ß1 treated with FSBA.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    FSBA 2F5/5 has been shown to detect Protein Kinase C-ß1 treated with FSBA.

  • 克隆

    单克隆

  • 克隆编号

    2F5/5

  • 骨髓瘤

    Sp2/0-Ag14

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab18575于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent dilution.
IP
Use at an assay dependent dilution.
说明
ELISA
Use at an assay dependent dilution.
IP
Use at an assay dependent dilution.

靶标

  • 相关性

    FSBA, a reactive ATP analogue, can be used to selectively label protein kinases, which may subsequently be detected and affinity purified using an anti-FSBA antibody.

  • 别名

    • reactive ATP analogue fluorylsulponylbenzoyladenosine antibody

文献 (0)

发表研究结果有使用 ab18575？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab18575 尚未被引用在任何文献中。

客户评价及客户问答

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Question

BATCH NUMBER 178694 ORDER NUMBER HC41681 DESCRIPTION OF THE PROBLEM Does not recognise anything. SAMPLE HeLa lysate. PRIMARY ANTIBODY FSBA antibody, Abcam, 1h @ 1:2500 DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Samples with no FSBA added. ANTIBODY STORAGE CONDITIONS Aliquoted and stored at -20. SAMPLE PREPARATION Basic lysis buffer with protease inhibitors stored at -80. AMOUNT OF PROTEIN LOADED 100 mcg. ELECTROPHORESIS/GEL CONDITIONS reducing, 10% TRANSFER AND BLOCKING CONDITIONS powdered milk at blocking agent, Tris Glycine transfer buffer. SECONDARY ANTIBODY [another company] goat anti-mouse HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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Answer

Thank you for your enquiry. This antibody has not been previously tested in Western blot. Thus, we cannot guarantee its suitability in this application. However, I would recommend trying this at a lower dilution, such as 1/1000. Since this is provided at a concentration of 0.9mg/ml, you are using less than 0.5ug/ml of antibody, which is rather low. I generally recommend using a few different concentrations between 0.5ug/ml and 5 ug/ml. I would also recommend incubating overnight at 4C. You did not mention your blocking conditions. I would recommend blocking with 5% milk in TBST for 1 h at room temperature, and incubating with the primary in TBST alone with no milk. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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