Anti-Frizzled 7抗体

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Frizzled 7 antibody (AB64636)

ab64636 staining Frizzled 7 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64636 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 7 antibody (AB64636)

IHC image of Frizzled 7 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64636, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Project

Western blot - Anti-Frizzled 7 antibody (AB64636)

Frizzled 7 has a predicted molecular weight of 64 kDa, however as it contains a number of potential glycosylation sites (SwissProt data) it is thought that this is the reason it is running at 90kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-Frizzled 7 antibody (ab64636) at 1 µg/mL

Lane 1:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Lane 2:

Spinal Cord (Rat) Tissue Lysate at 10 µg

Lane 3:

Human brain tissue lysate - total protein (<a href='/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 4:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 64 kDa

Observed band size: 90 kDa

true

Exposure time: 10min

• WB

Unknown

Western blot - Anti-Frizzled 7 antibody (AB64636)

All lanes:

Western blot - Anti-Frizzled 7 antibody (ab64636) at 1 µg/mL

Lane 1:

Skeletal Muscle (Rat) Tissue Lysate at 10 µg

Lane 2:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 3:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

Predicted band size: 64 kDa

Observed band size: 85 kDa

false

• IHC-P

AbReview18449****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 7 antibody (AB64636)

ab64636 (1/250) staining Frizzled 7 in paraffin-embedded Human kidney (papillary cancer) tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.

This image is courtesy of an abreview submitted by Antibody Solutions Ltd.