Anti-Frizzled 5抗体(ab75234)

Thank you for your patience in this matter.
I have been in contact with the lab about ab75234 and we are having the peptide used to generate the antibody added to the catalogue. That way I can send it to you to use as a blocking peptide and determine if the signal you are getting is specific or not. The only problem, is that it will take 3 weeks to have the peptide added to the catalogue, which I understand is a long delay.
If you cannot wait that long, please let me know and I will be happy to look into either replacing or refunding the cost of the antibody for you.
I look forward to your reply and helping resolve this issue.

Thank you for your reply..
Firstly, I would like to assure you that the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.
I would just like to ask a few more questions that may help me solve the issue that you are having:
1 - Does the band of interest (or potential band of interest) decrease when you add your shRNA? I am just trying to determine if the antibody is actually detecting the correct band.
2 - Have you tried any other blocking reagents, other than milk? Possible 5% BSA?
Thank you for your patience in this matter and I look forward to your reply and helping resolve this issue for you.

Thank you for contacting Abcam.
I have attached your image, with an arrow pointing to the band that I think is Frizzled 5. However is the banding pattern that you were expecting to see between your samples?
I would be more than happy to help try to clear up the number of bands that the antibody is detecting. To do this, would you be able to answer a few questions about your protocol:
1 - What sample type are you using?
2 - How much sample are you loading?
3 - What type of blocking agent are you using?
4 - What primary antibody concentrations have you tried and how long do you incubate for?
I look forward to your reply.

Thank you for contacting us last week. Unfortunately we do not stock the immunogen peptide for ab75234 (anti-frizzled 5 antibody). Apologies for any inconvenience.

Thank you for your reply. I am sorry to confirm that ab58177 been tested on recombinant protein only, and not on endogenous samples. Therefore, as requested,  I have asked our Finance department to issue a credit note for you. Credit note ID: 18393   The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice  

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Thank you for providing the data in relation to this problem. This lets us understand the problem more fully and allows us to  investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. In order to help with this further could you please fill out the questionaire that I have attached. It should only take 5-10 minutes. Could you also let me know the sequence used for the fusion protein you are expressing. You can send a plasmid map if its easier. Unfortunately I was unable to find the order which you refered to. Was the antibody purchased through a distributor? Could you please supply me with an order date and delivery address to help me locate the order. I appreciate the time spent in the lab and the dissapointment experienced with this antibody. I will do my best to resolve this matter as quickly as I can. Many thanks for your cooperation in this matter.