重组Anti-FPGS抗体[EPR11064] (ab184564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11064] to FPGS
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FPGS抗体[EPR11064]
参阅全部 FPGS 一抗 -
描述
兔单克隆抗体[EPR11064] to FPGS -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- JAR, HeLa and K562 cell lysates. HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11064 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184564于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
|
说明 |
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Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Conversion of folates to polyglutamate derivatives. This allows tissues to concentrate folate at higher levels than in plasma. -
通路
Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis. -
序列相似性
Belongs to the folylpolyglutamate synthase family. -
细胞定位
Mitochondrion. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2356 Human
- Entrez Gene: 14287 Mouse
- Entrez Gene: 687266 Rat
- Omim: 136510 Human
- SwissProt: Q05932 Human
- SwissProt: P48760 Mouse
- Unigene: 335084 Human
- Unigene: 3830 Mouse
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别名
- EC 6.3.2.17 antibody
- FOLC antibody
- FOLC_HUMAN antibody
see all
图片
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Anti-FPGS antibody [EPR11064] (ab184564) at 1/50000 dilution + K562 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa -
All lanes : Anti-FPGS antibody [EPR11064] (ab184564) at 1/10000 dilution
Lane 1 : JAR cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Intracellular Flow Cytometry analysis ofFPGS expression in paraformaldehyde-fixed HeLa cells using ab184564 at 1/60 dilution (red) and a rabbit IgG as negative control (green). Secondary antibody: Goat anti-rabbit IgG (FITC).
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab184564 被引用在 2 文献中.
- Oiwa K et al. Characterization of newly established Pralatrexate-resistant cell lines and the mechanisms of resistance. BMC Cancer 21:879 (2021). PubMed: 34332580
- Srivastava AC et al. Elimination of human folypolyglutamate synthetase alters programming and plasticity of somatic cells. FASEB J 33:13747-13761 (2019). PubMed: 31585510