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Anti-FOXP3抗体[mAbcam 22510] (ab22510)

Reviews (8)Q&A (1)References (122)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
  • Western blot - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)

Key features and details

  • Mouse monoclonal [mAbcam 22510] to FOXP3
  • Suitable for: IHC-Fr, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

HRP

概述

  • 产品名称

    Anti-FOXP3抗体[mAbcam 22510]
    参阅全部 FOXP3 一抗

  • 描述

    小鼠单克隆抗体[mAbcam 22510] to FOXP3

  • 宿主

    Mouse

  • 特异性

    This product detects an epitope near the C-terminus of the FOXP3 protein and is expected to detect full length FOXP3. It is not expected to detect the cleaved forms of FOXP3.

  • 经测试应用

    适用于: IHC-Fr, IHC-P, WBmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human tonsil and spleen tissue. Human colon carcinoma tissue. IHC-Fr: Human tonsil tissue. WB: HEK293 cells overexpressing human FOXP3.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab22510于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use a concentration of 10 µg/ml.
IHC-P (5)
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
说明
IHC-Fr
Use a concentration of 10 µg/ml.
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Probable transcription factor. Plays a critical role in the control of immune response.

  • 疾病相关

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

  • 序列相似性

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)

      IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)

      IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

       

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)

      IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      Western blot - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      All lanes : Anti-FOXP3 antibody [mAbcam 22510] (ab22510) at 1 µg/ml

      Lane 1 : HEK293 cells overexpressing human FOXP3
      Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 48 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 45 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)This image is courtesy of Alison Banham and Bridget Fox, University of Oxford

      Clone mAbcam 22510 used to stain formalin-fixed paraffin-embedded sections of human tonsil. Antigens were heat retrieved with pH9 Tris-EDTA buffer.

      These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
      IHC image of ab22510 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22510, 20µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    文献 (122)

    发表研究结果有使用 ab22510？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab22510 被引用在 122 文献中.

    • Cui B  et al. Dendritic cells originating exosomal miR-193b-3p induces regulatory T cells to alleviate liver transplant rejection. Int Immunopharmacol 114:109541 (2023). PubMed: 36700764
    • Cui S  et al. Tracking the evolution of esophageal squamous cell carcinoma under dynamic immune selection by multi-omics sequencing. Nat Commun 14:892 (2023). PubMed: 36807354
    • Maccarinelli F  et al. Iron supplementation enhances RSL3-induced ferroptosis to treat naïve and prevent castration-resistant prostate cancer. Cell Death Discov 9:81 (2023). PubMed: 36872341
    • Knebel M  et al. Prognostic impact of intra- and peritumoral immune cell subpopulations in head and neck squamous cell carcinomas - comprehensive analysis of the TCGA-HNSC cohort and immunohistochemical validation on 101 patients. Front Immunol 14:1172768 (2023). PubMed: 37383237
    • Deng Y  et al. HBx promotes hepatocellular carcinoma progression by repressing the transcription level of miR-187-5p. Aging (Albany NY) 15:7533-7550 (2023). PubMed: 37531206
    View all Publications for this product

    客户评价及客户问答

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    Western blot abreview for Anti-FOXP3 antibody [mAbcam 22510]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain, Embryonic body)
    Loading amount
    20 µg
    Specification
    Brain, Embryonic body
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    提交于 May 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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