Anti-FOXP2抗体(ab16046)
Key features and details
- Rabbit polyclonal to FOXP2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-FOXP2抗体
参阅全部 FOXP2 一抗 -
描述
兔多克隆抗体to FOXP2 -
宿主
Rabbit -
特异性
From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse
预测可用于: Rat
不与反应:
Human -
免疫原
Synthetic peptide corresponding to Human FOXP2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16278) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/1000.
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| IHC-P | (4) |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/1000. |
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language. -
组织特异性
Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung. -
疾病相关
Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2). -
序列相似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
发展阶段
Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber. -
结构域
The leucine-zipper is required for dimerization and transcriptional repression. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 114142 Mouse
- Entrez Gene: 500037 Rat
- SwissProt: P58463 Mouse
- SwissProt: P0CF24 Rat
- Unigene: 332919 Mouse
- Unigene: 225249 Rat
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别名
- CAG repeat protein 44 antibody
- CAGH44 antibody
- DKFZp686H1726 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)
IHC image of FOXP2 staining in mouse e17 foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16046, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)This image is courtesy of an abreview submitted by Jeremy Dasen.Mouse spinal cord was fixed in paraformaldehyde, blocked in 1% BSA for 30 minutes then incubated with ab16046 at 1/8000 dilution for 18 hours. This image was submitted as part of a review by Jeremy Dasen.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (178)
ab16046 被引用在 178 文献中.
- Yan L et al. FOXP2 suppresses the proliferation, invasion, and aerobic glycolysis of hepatocellular carcinoma cells by regulating the KDM5A/FBP1 axis. Environ Toxicol 39:341-356 (2024). PubMed: 37713600
- Sterling NA et al. P53 independent pathogenic mechanisms contribute to BubR1 microcephaly. Front Cell Dev Biol 11:1282182 (2023). PubMed: 37900274
- Lischinsky JE et al. Transcriptionally defined amygdala subpopulations play distinct roles in innate social behaviors. Nat Neurosci 26:2131-2146 (2023). PubMed: 37946049
- Tang L et al. A primate nigrostriatal atlas of neuronal vulnerability and resilience in a model of Parkinson's disease. Nat Commun 14:7497 (2023). PubMed: 37980356
- Rodríguez-Urgellés E et al. Thalamic Foxp2 regulates output connectivity and sensory-motor impairments in a model of Huntington's Disease. Cell Mol Life Sci 80:367 (2023). PubMed: 37987826