重组Anti-FOXP1抗体[EPR4113] (ab134055)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4113] to FOXP1
- Suitable for: ChIP-sequencing, WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXP1抗体[EPR4113]
参阅全部 FOXP1 一抗 -
描述
兔单克隆抗体[EPR4113] to FOXP1 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: ChIP-sequencing, WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human FOXP1 aa 350-450. The exact sequence is proprietary.
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阳性对照
- Daudi, MCF7, T47D, 2.4G2, RAW264.7, (Rat B cell lymphoma B lymphocyte) whole cell lysates, and Jurkat whole cell lysate (ab7899); mouse lung tissue lysates, Human tonsil tissue, Human diffuse large B-cell lymphoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4113 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 647 Anti-FOXP1 antibody [EPR4113] (ab196977)
- Alexa Fluor® 488 Anti-FOXP1 antibody [EPR4113] (ab197522)
- Anti-FOXP1 antibody [EPR4113] - BSA and Azide free (ab248661)
- APC Anti-FOXP1 antibody [EPR4113] (ab319322)
- PE Anti-FOXP1 antibody [EPR4113] (ab319460)
- Alexa Fluor® 594 Anti-FOXP1 antibody [EPR4113] (ab319814)
- Alexa Fluor® 555 Anti-FOXP1 antibody [EPR4113] (ab319955)
- Alexa Fluor® 750 Anti-FOXP1 antibody [EPR4113] (ab321070)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134055于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP-sequencing |
Use 8µg for 107 cells.
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WB |
1/1000 - 1/10000. Detects a band of approximately 50-95 kDa (predicted molecular weight: 75 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified format use at 1/250-1/500 dilution. |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/1000.
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说明 |
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ChIP-sequencing
Use 8µg for 107 cells. |
WB
1/1000 - 1/10000. Detects a band of approximately 50-95 kDa (predicted molecular weight: 75 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified format use at 1/250-1/500 dilution. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
疾病相关
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
序列相似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
结构域
The leucine-zipper is required for dimerization and transcriptional repression. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Omim: 605515 Human
- SwissProt: Q9H334 Human
- SwissProt: P58462 Mouse
- SwissProt: Q498D1 Rat
- Unigene: 431498 Human
see all -
别名
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
图片
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Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/5000 dilution (Purified) + 2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 75 kDa
Observed band size: 50,70,95 kDa why is the actual band size different from the predicted?The multiple bands are consistent with what have been described in the literature PMID: 12692134.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human diffuse large B-cell lymphoma tissue sections labeling FOXP1 with purified ab134055 at 1/1000 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/1000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : Mouse lung cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 75 kDa
Observed band size: 50,70,95 kDa why is the actual band size different from the predicted?The multiple bands are consistent with what have been described in the literature PMID: 12692134.
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Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FOXP1 with purified ab134055 at 1/500 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134055 (unpurified) showing +ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab134055 (unpurified) showing +ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling FOXP1 with ab134055 (unpurified) at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab134055 (unpurified) showing +ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134055 (unpurified) showing -ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab134055 (unpurified) showing +ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of Daudi (human Burkitt's lymphoma) cells labeling FOXP1 with purified ab134055 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab134055 (unpurified) showing +ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)
Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab134055 (unpurified) showing -ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab134055 被引用在 6 文献中.
- Tan Y et al. Low-dose exercise protects the heart against established myocardial infarction via IGF-1-upregulated CTRP9 in male mice. MedComm (2020) 4:e411 (2023). PubMed: 38020715
- Xia X et al. LncRNA XIST promotes carboplatin resistance of ovarian cancer through activating autophagy via targeting miR-506-3p/FOXP1 axis. J Gynecol Oncol 33:e81 (2022). PubMed: 36335987
- Shen W et al. CircFOXP1/FOXP1 promotes osteogenic differentiation in adipose-derived mesenchymal stem cells and bone regeneration in osteoporosis via miR-33a-5p. J Cell Mol Med 24:12513-12524 (2020). PubMed: 32996692
- Rodenas-Cuadrado PM et al. Mapping the distribution of language related genes FoxP1, FoxP2, and CntnaP2 in the brains of vocal learning bat species. J Comp Neurol 526:1235-1266 (2018). IHC-P . PubMed: 29297931
- Louis Sam Titus ASC et al. Reduced Expression of Foxp1 as a Contributing Factor in Huntington's Disease. J Neurosci 37:6575-6587 (2017). PubMed: 28550168
- Richner M et al. MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons. Nat Protoc 10:1543-55 (2015). PubMed: 26379228