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重组RabMAb

重组Anti-FOXO4/AFX (phospho S262)抗体[EPR2315(2)] (ab126594)

Submit a review Submit a question References (5)
Western blot - Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
  • OI-RD Scanning - Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
  • Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2315(2)] to FOXO4/AFX (phospho S262)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-FOXO4/AFX (phospho S262)抗体[EPR2315(2)]
    参阅全部 FOXO4/AFX 一抗

  • 描述

    兔单克隆抗体[EPR2315(2)] to FOXO4/AFX (phospho S262)

  • 宿主

    Rabbit

  • 特异性

    This antibody only detects FOXO4 / AFX phosphorylated at serine 262.

  • 经测试应用

    适用于: WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • FOXO4/AFX transfected 293T cell lysate.

  • 常规说明

    This antibody was developed as part of a collaboration between the University of Utrecht, Netherlands and the lab of Tobias Dansen.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126594于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).
说明
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

  • 组织特异性

    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

  • 疾病相关

    Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.

  • 序列相似性

    Contains 1 fork-head DNA-binding domain.

  • 翻译后修饰

    Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.

  • 细胞定位

    Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AFX antibody
      • AFX1 antibody
      • Afxh antibody
      • ALL1-fused gene from X chromosome antibody
      • Fork head domain transcription factor AFX1 antibody
      • Forkhead box O4 antibody
      • Forkhead box protein O4 antibody
      • FOXO 4 antibody
      • Foxo4 antibody
      • FOXO4_HUMAN antibody
      • MGC117660 antibody
      • MGC120490 antibody
      • Mixed lineage leukemia, translocated to, 7 antibody
      • MLLT7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
      • RGD1561201 antibody
      see all

    图片

    • Western blot - Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
      Western blot - Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
      All lanes : Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594) at 1/10000 dilution

      Lane 1 : Cell lysate from FOXO4 transfected 293T
      Lane 2 : Cell lysate from FOXO4 transfected 293T treated with Alkaline Phosphatase
      Lane 3 : Cell lysate from non-transfected 293T

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa

    • OI-RD Scanning - Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
      OI-RD Scanning - Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)
      Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)

    文献 (5)

    发表研究结果有使用 ab126594？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab126594 被引用在 5 文献中.

    • Zhao Y  et al. mTORC2 orchestrates monocytic and granulocytic lineage commitment by an ATF5-mediated pathway. iScience 26:107540 (2023). PubMed: 37649699
    • Lu W  et al. Resveratrol inhibits bile acid-induced gastric intestinal metaplasia via the PI3K/AKT/p-FoxO4 signalling pathway. Phytother Res 35:1495-1507 (2021). PubMed: 33103284
    • Sun X  et al. LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1a-FOXO4-p21-dependent signaling axis. EMBO Rep 21:e48796 (2020). PubMed: 32149459
    • Zhang F  et al. Oncogenic RAS-induced CK1a drives nuclear FOXO proteolysis. Oncogene 37:363-376 (2018). WB . PubMed: 28945225
    • Mandai S  et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep 8:9101 (2018). PubMed: 29904119

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