Key features and details
- Rabbit polyclonal to FOXO4/AFX (phospho S197)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
产品名称Anti-FOXO4/AFX (phospho S197)抗体
参阅全部 FOXO4/AFX 一抗
描述兔多克隆抗体to FOXO4/AFX (phospho S197)
经测试应用适用于: ICC/IF, WB, IHC-Pmore details
Synthetic peptide corresponding to Human FOXO4/AFX aa 150-250 (phospho S197). derived from human FOXO4/AFX around the phosphorylation site of serine 197 (A-A-SP-M-D)
Database link: P98177
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
纯度Immunogen affinity purified
纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The Abpromise guarantee
IHC: 1/50 - 1/100.
ICC/IF: Use at a concentration of 1-5 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
疾病相关Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
序列相似性Contains 1 fork-head DNA-binding domain.
翻译后修饰Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
细胞定位Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
- Information by UniProt
- AFX antibody
- AFX1 antibody
- Afxh antibody
All lanes : Anti-FOXO4/AFX (phospho S197) antibody (ab47278) at 1/500 dilution
Lane 1 : Extracts from 293 cells.
Lane 2 : Extracts from 293 cells. Immunizing peptide 1ug/mL
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Peptide - +
This image shows human breast carcinoma stained with ab47781 at 1/50 dilution. Right hand image:tissue was treated with immunogenic peptide, left hand image: untreated.
ICC/IF image of ab47278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.