重组Anti-FOXO4/AFX抗体[EPR5442] (ab128908)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5442] to FOXO4/AFX
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXO4/AFX抗体[EPR5442]
参阅全部 FOXO4/AFX 一抗 -
描述
兔单克隆抗体[EPR5442] to FOXO4/AFX -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human FOXO4/AFX aa 450-550 (C terminal). The exact sequence is proprietary.
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阳性对照
- 293T, fetal heart, fetal muscle, fetal lung, and HuT-78 lysates. Permeabilized 293T cells.
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常规说明
This antibody was developed as part of a collaboration between the University of Utrecht in the Netherlands and the lab of Tobias Dansen.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5442 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128908于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).
|
IP |
1/10 - 1/100.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa). |
IP
1/10 - 1/100. |
靶标
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功能
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. -
组织特异性
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. -
疾病相关
Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. -
细胞定位
Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4303 Human
- Entrez Gene: 54601 Mouse
- Entrez Gene: 302415 Rat
- Omim: 300033 Human
- SwissProt: P98177 Human
- SwissProt: Q9WVH3 Mouse
- Unigene: 584654 Human
- Unigene: 240299 Mouse
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别名
- AFX antibody
- AFX1 antibody
- Afxh antibody
see all
图片
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All lanes : Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : Fetal heart lysate
Lane 3 : Fetal muscle lysate
Lane 4 : Fetal lung lysate
Lane 5 : HuT-78 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of FOXO4/AFX in permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells using ab128908 at 1/100 dilution (Red) compared to a non-specific negative control antibody (green).
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Purified ab128908 at 1/100 dilution (2µg) immunoprecipitating FOXO4/AFX in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab128908 + 293T whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128908 in 293T whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 65 kDa -
All lanes : Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution
Lane 1 : 293T transfected with FOXO4/AFX
Lane 2 : 293T cell lysate
Secondary
Lane 1 : Goat-anti-rabbit HRP at 1/2000 dilution
Lane 2 : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab128908 被引用在 21 文献中.
- Galley JC et al. FoxO4 controls sGCβ transcription in vascular smooth muscle. Am J Physiol Heart Circ Physiol 322:H417-H426 (2022). PubMed: 35089807
- Wu J et al. Interleukin‑Iβ promotes cartilage degeneration by regulating forkhead box protein O4 and type Ⅱ collagen. Mol Med Rep 24:N/A (2021). PubMed: 34549304
- Lee SH et al. FBXO7 triggers caspase 8-mediated proteolysis of the transcription factor FOXO4 and exacerbates neuronal cytotoxicity. J Biol Chem 297:101426 (2021). PubMed: 34800438
- He L et al. Transcriptional activation of ENPP2 by FoxO4 protects cardiomyocytes from doxorubicin-induced toxicity. Mol Med Rep 24:N/A (2021). PubMed: 34296293
- Zhang J et al. Exosomal-miR-1184 derived from mesenchymal stem cells alleviates cisplatin-associated acute kidney injury. Mol Med Rep 24:N/A (2021). PubMed: 34515319