重组Anti-FOXO4/AFX抗体[EPR2150(3)] (ab126757)
![Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.cn/ps/products/126/ab126757/Images/ab126757-140782-anti-foxo4-afx-antibody-epr21503-western-blot.jpg "Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXO4/AFX抗体[EPR2150(3)]
参阅全部 FOXO4/AFX 一抗 -
描述
兔单克隆抗体[EPR2150(3)] to FOXO4/AFX -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human FOXO4/AFX. The exact sequence is proprietary.
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阳性对照
- HA-FOXO4/AFX transfected 293T cell lysate; HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate; Human placenta tissue.
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常规说明
This antibody was developed as part of a collaboration between Epitomics, the University of Utrecht, Netherlands and the lab of Tobias Dansen.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.81 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2150(3) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126757于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.)
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.) |
应用说明
Is unsuitable for IP.
靶标
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功能
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. -
组织特异性
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. -
疾病相关
Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. -
细胞定位
Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4303 Human
- Omim: 300033 Human
- SwissProt: P98177 Human
- Unigene: 584654 Human
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别名
- AFX antibody
- AFX1 antibody
- Afxh antibody
see all
图片
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Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)All lanes : Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757) at 1/10000 dilution
Lane 1 : HA-FOXO4/AFX transfected 293T cell lysate
Lane 2 : HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.cn/ps/products/126/ab126757/Images/ab126757-140781-anti-foxo4-afx-antibody-epr21503-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)ab126757, at 1/100 dilution, staining FOXO4/AFX in paraffin-embedded Human placenta tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.cn/ps/products/126/ab126757/Images/ab126757-140780-anti-foxo4-afx-antibody-epr21503.jpg)
OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757) -
![Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.cn/ps/products/126/ab126757/Images/ab126757-4-benefits-of-recombinant-antibodies.png)
Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab126757 被引用在 1 文献中.
- Huang P et al. Correlations of ALD, Keap-1, and FoxO4 expression with traditional tumor markers and clinicopathological characteristics in colorectal carcinoma. Medicine (Baltimore) 101:e30222 (2022). PubMed: 36042628