Anti-FOXO3A (phospho S253)抗体(ab47285)
Key features and details
- Rabbit polyclonal to FOXO3A (phospho S253)
- Suitable for: WB, ELISA, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FOXO3A (phospho S253)抗体
参阅全部 FOXO3A 一抗 -
描述
兔多克隆抗体to FOXO3A (phospho S253) -
宿主
Rabbit -
经测试应用
适用于: WB, ELISA, IHC-P, ICC/IF, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human FOXO3A (phospho S253).
Database link: O43524 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/500 - 1/1000. Detects a band of approximately 67, 105 kDa (predicted molecular weight: 71 kDa).
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
See Abreview. |
| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 67, 105 kDa (predicted molecular weight: 71 kDa). |
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ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-Fr
Use at an assay dependent concentration. See Abreview. |
靶标
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功能
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
细胞定位
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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数据库链接
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 294515 Rat
- Omim: 602681 Human
- SwissProt: O43524 Human
- SwissProt: Q9WVH4 Mouse
- Unigene: 220950 Human
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别名
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
图片
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Western blot - Anti-FOXO3A (phospho S253) antibody (ab47285)All lanes : Anti-FOXO3A (phospho S253) antibody (ab47285) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells untreated
Lane 2 : extracts from NIH/3T3 cells treated with serum
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
Observed band size: 105,67 kDa why is the actual band size different from the predicted?
Serum treatment:-
1. Add fresh basic medium to the vessels and starve the cells for 18-24 hours.
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2. Drain the cultured medium, add growth medium and basic medium containing 10% serum to the vessels.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody (ab47285)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47285 at a 1/50 dilution.Left image : Untreated
Right image : Treated with P-Peptide
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A (phospho S253) antibody (ab47285)ICC/IF image of ab47285 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A (phospho S253) antibody (ab47285)This image is courtesy of an anonymous Abreview.ab47285 at 1/200 dilution staining FOXO3A in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permebilized in 0.5% Triton X-100 and then blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution. Image show green staining with ab47285 and blue with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (51)
ab47285 被引用在 51 文献中.
- Bordbari S et al. SIRT1-mediated deacetylation of FOXO3a transcription factor supports pro-angiogenic activity of interferon-deficient tumor-associated neutrophils. Int J Cancer 150:1198-1211 (2022). PubMed: 34751438
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Yeh CC et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep 25:N/A (2022). PubMed: 34913071
- Peng M et al. Programmed death-ligand 1 signaling and expression are reversible by lycopene via PI3K/AKT and Raf/MEK/ERK pathways in tongue squamous cell carcinoma. Genes Nutr 17:3 (2022). PubMed: 35164673
- Polvani S et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189