Anti-FOXO3A抗体(ab70314)
Key features and details
- Rabbit polyclonal to FOXO3A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FOXO3A抗体
参阅全部 FOXO3A 一抗 -
描述
兔多克隆抗体to FOXO3A -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residues 250 and 300 of human FOXO3A. (Swiss-Prot O43524)
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阳性对照
- Whole cell lysate from MCF7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/15000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/5000 - 1/15000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
细胞定位
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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数据库链接
- Entrez Gene: 535530 Cow
- Entrez Gene: 481954 Dog
- Entrez Gene: 100066558 Horse
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 733621 Pig
- Omim: 602681 Human
- SwissProt: O43524 Human
see all -
别名
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
图片
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Western blot - Anti-FOXO3A antibody (ab70314)All lanes : Anti-FOXO3A antibody (ab70314) at 0.4 µg/ml
Lane 1 : 293T cell Lysate
Lane 2 : Jurkat cell Lysate
Lane 3 : Mouse TCMK cell Lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FOXO3A antibody (ab70314)Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg.
Lane 2: MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3:MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Western Blot carried out using ab70314 at 1ug/ml
Decction: ECL for 3 minutes.
All lanes : Anti-FOXO3A antibody (ab70314) at 1 µg/ml
Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg
Lane 2 : MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3 : MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Developed using the ECL technique.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab70314)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70314 at 1/500 (2µg/ml). Detection: DAB.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab70314 被引用在 1 文献中.
- Wang Y et al. miR-155 promotes fibroblast-like synoviocyte proliferation and inflammatory cytokine secretion in rheumatoid arthritis by targeting FOXO3a. Exp Ther Med 19:1288-1296 (2020). PubMed: 32010301