重组Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26)抗体(ab128876)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2314(2)] to FOXO1A (phospho T24) + FOXO4/AFX (phospho T28) + FOXO3A (phospho T32) + Foxo6 (phospho T26)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26)抗体 -
描述
兔单克隆抗体[EPR2314(2)] to FOXO1A (phospho T24) + FOXO4/AFX (phospho T28) + FOXO3A (phospho T32) + Foxo6 (phospho T26) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293T transfected with HA-FOXO4/AFX
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常规说明
This antibody was developed as part of a collaboration with Tobais Dansen from the University of Utrecht in the Netherlands.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数(KD)
KD = 2.51 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2314(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
靶标
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细胞定位
FOXO1A: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOXO4/AFX: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. FOXO3A: Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Foxo6: Cytoplasmic and Nuclear. When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors. -
数据库链接
- Entrez Gene: 100132074 Human
- Entrez Gene: 2308 Human
- Entrez Gene: 2309 Human
- Entrez Gene: 4303 Human
- Omim: 136533 Human
- Omim: 300033 Human
- Omim: 602681 Human
- Omim: 611457 Human
see all
图片
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All lanes : Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (ab128876) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : 293T transfected with HA-FOXO4/AFX cell lysates
Lane 3 : 293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates
Predicted band size: 53 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab128876 尚未被引用在任何文献中。