Anti-FOXO1A (phospho S319)抗体(ab47326)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S319)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FOXO1A (phospho S319)抗体
参阅全部 FOXO1A 一抗 -
描述
兔多克隆抗体to FOXO1A (phospho S319) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human FOXO1A aa 250-350 (phospho S319).
Database link: Q12778 -
阳性对照
- human breast carcinoma, HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47326于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
组织特异性
Ubiquitous. -
疾病相关
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2308 Human
- Entrez Gene: 56458 Mouse
- Entrez Gene: 84482 Rat
- Omim: 136533 Human
- SwissProt: Q12778 Human
- SwissProt: Q9R1E0 Mouse
- SwissProt: G3V7R4 Rat
- Unigene: 370666 Human
see all -
别名
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
see all
图片
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All lanes : Anti-FOXO1A (phospho S319) antibody (ab47326)
Lane 1 : extracts from EGF treated HeLa cells
Lane 2 : extracts from HeLa cells
Predicted band size: 70 kDa
Typically 5-30ug of total protein was loaded per lane of the gel. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A (phospho S319) antibody (ab47326)ab47326 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
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ICC/IF image of ab47326 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab47326 被引用在 2 文献中.
- Cheng L et al. SGK2 promotes prostate cancer metastasis by inhibiting ferroptosis via upregulating GPX4. Cell Death Dis 14:74 (2023). PubMed: 36720852
- Nihei S et al. Bevacizumab Increases Endothelin-1 Production via Forkhead Box Protein O1 in Human Glomerular Microvascular Endothelial Cells In Vitro. Int J Nephrol 2021:8381115 (2021). PubMed: 34912580