Anti-FOXN3抗体(ab129453)
Key features and details
- Rabbit polyclonal to FOXN3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FOXN3抗体
参阅全部 FOXN3 一抗 -
描述
兔多克隆抗体to FOXN3 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to Human FOXN3 aa 440-490.
Database link: NP_001078940.1 -
阳性对照
- WB: Jurkat, HEK-293T and HeLa whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
pH: 7-8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129453于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 54 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints). -
序列相似性
Contains 1 fork-head DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1112 Human
- Entrez Gene: 733584 Pig
- Omim: 602628 Human
- SwissProt: O00409 Human
- SwissProt: Q33BP8 Pig
- Unigene: 434286 Human
- Unigene: 621371 Human
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别名
- C14orf116 antibody
- Checkpoint suppressor 1 antibody
- CHES1 antibody
see all
图片
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Western blot - Anti-FOXN3 antibody (ab129453)All lanes : Anti-FOXN3 antibody (ab129453) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FOXN3 antibody (ab129453)Detection of FOXN3 by Western Blot of Immunprecipitate.
Lane 1: ab129453 at 1µg/ml staining FOXN3 in HeLa whole cell lysate immunoprecipitated using ab129453 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab129453 被引用在 7 文献中.
- Zhu X et al. p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway. Nucleic Acids Res 51:2195-2214 (2023). PubMed: 36794705
- Qin M et al. DNA methylation abnormalities induced by advanced maternal age in villi prime a high-risk state for spontaneous abortion. Clin Epigenetics 15:44 (2023). PubMed: 36945044
- Xu Z et al. Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer. Cell Death Discov 8:474 (2022). PubMed: 36450706
- He H et al. Novel tumor-suppressor FOXN3 is downregulated in adult acute myeloid leukemia. Oncol Lett 18:1521-1529 (2019). PubMed: 31423219
- Xu Z et al. Checkpoint suppressor 1 suppresses transcriptional activity of ERa and breast cancer cell proliferation via deacetylase SIRT1. Cell Death Dis 9:559 (2018). PubMed: 29752474
- Sun M et al. MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/ß-catenin pathway. Cell Biol Int N/A:N/A (2018). PubMed: 29972255
- Li W et al. The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. J Clin Invest 127:3421-3440 (2017). PubMed: 28805661