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重组RabMAb

重组Anti-FOXL2抗体[EPR23523-68] - BSA and Azide free (ab272050)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
  • Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23523-68] to FOXL2 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-FOXL2抗体[EPR23523-68] - BSA and Azide free
    参阅全部 FOXL2 一抗

  • 描述

    兔单克隆抗体[EPR23523-68] to FOXL2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-P, IHC-Fr, IPmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse ovary, Rat ovary, Rat testis and K-562, Human ovary lysates. IHC-P: Mouse ovary, Rat ovary and Human ovary, Mouse spleen tissues. IHC-Fr: Mouse ovary tissue. IP: Mouse ovary cell lysate. ICC/IF: NIH/3T3 and K-562 cells.

  • 常规说明

    ab252050 is the carrier-free version of ab246511.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272050于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).

We do not recommend this antibody for WB using human samples.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).

We do not recommend this antibody for WB using human samples.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.

  • 组织特异性

    In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.

  • 疾病相关

    Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
    Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

  • 序列相似性

    Contains 1 fork-head DNA-binding domain.

  • 翻译后修饰

    Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Blepharophimosis antibody
      • Blepharophimosis epicanthus inversus and ptosis 1 antibody
      • Blepharophimosis epicanthus inversus and ptosis antibody
      • BPES 1 antibody
      • BPES antibody
      • BPES1 antibody
      • Epicanthus inversus and ptosis 1 antibody
      • Forkhead box L2 antibody
      • Forkhead box protein L2 antibody
      • Forkhead transcription factor FOXL2 antibody
      • FOX L2 antibody
      • FOXL 2 antibody
      • FOXL2 antibody
      • FOXL2_HUMAN antibody
      • PFRK antibody
      • PINTO antibody
      • PITUITARY FORKHEAD FACTOR antibody
      • POF 3 antibody
      • POF3 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on granulosa cells and some other cells in mouse ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunohistochemistry (Frozen sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized Mouse ovary tissue labeling FOXL2 with ab246511 at 1/250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Nuclear staining on  mouse ovary. The nuclear counter stain is DAPI (blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in NIH/3T3 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunocytochemistry/ Immunofluorescence - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblasts) cells labelling FOXL2 with primary antibody anti-FOXL2 (ab246511) at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing mainly nuclear staining in K-562 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunoprecipitation - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      FOXL2 was immunoprecipitated from 0.35 mg Mouse ovary tissue lysate with ab246511 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse ovary tissue lysate 10ug

      Lane 2: ab246511 IP in Mouse ovary tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246511 in mouse ovary tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 84 seconds

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on granulosa cells and some other cells in rat ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human ovary. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling FOXL2 with ab246511 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen. The section was incubated with ab246511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246511).

    • Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)
      Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free (ab272050)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab272050？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272050 被引用在 1 文献中.

    • Aryan A  et al. Methamphetamine can induce alteration of histopathology and sex determination gene expression through the oxidative stress pathway in the testes of human post-mortem. Andrologia 54:e14441 (2022). PubMed: 35428984

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