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Anti-FOXL2抗体[262C1a] (ab58622)

Reviews (2)Q&A (2)
Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)

    Key features and details

    • Mouse monoclonal [262C1a] to FOXL2
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-FOXL2抗体[262C1a]
      参阅全部 FOXL2 一抗

    • 描述

      小鼠单克隆抗体[262C1a] to FOXL2

    • 宿主

      Mouse

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Recombinant fragment

    • 免疫原

      Recombinant fragment: NSIRHNLSLN ECFIKVPREG GGERKGNYWT LDPACEDMFE KGNYRRRRRM KRPFRPPPAH FQPGKGLFGA GGAAGGCGVA GAGADGYGYL APPKYLQSGF LN, corresponding to amino acids 100-201 of Human FOXL2

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • 存储溶液

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 纯化说明

      Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

    • 克隆

      单克隆

    • 克隆编号

      262C1a

    • 同种型

      IgG1

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab58622于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
    说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

    靶标

    • 功能

      Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.

    • 组织特异性

      In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.

    • 疾病相关

      Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
      Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

    • 序列相似性

      Contains 1 fork-head DNA-binding domain.

    • 翻译后修饰

      Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 别名

      • Blepharophimosis antibody
      • Blepharophimosis epicanthus inversus and ptosis 1 antibody
      • Blepharophimosis epicanthus inversus and ptosis antibody
      • BPES 1 antibody
      • BPES antibody
      • BPES1 antibody
      • Epicanthus inversus and ptosis 1 antibody
      • Forkhead box L2 antibody
      • Forkhead box protein L2 antibody
      • Forkhead transcription factor FOXL2 antibody
      • FOX L2 antibody
      • FOXL 2 antibody
      • FOXL2 antibody
      • FOXL2_HUMAN antibody
      • PFRK antibody
      • PINTO antibody
      • PITUITARY FORKHEAD FACTOR antibody
      • POF 3 antibody
      • POF3 antibody
      see all

    图片

    • Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)
      Western blot - Anti-FOXL2 antibody [262C1a] (ab58622)
      Anti-FOXL2 antibody [262C1a] (ab58622) + immunogen (recombinant fragment)

      Predicted band size: 42 kDa



      The low MW of the band is due to the fact that the immunising recombinant fragment, rather than the full lenght protein, was used as positive control.

    文献 (0)

    发表研究结果有使用 ab58622？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab58622 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FOXL2 antibody [262C1a]

     Poor
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Turtle Cell (Gonad)
    Specification
    Gonad
    Fixative
    paraformaldehyde and methanol
    Permeabilization
    Yes - 0.2% triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 29 2009

    Western blot abreview for Anti-FOXL2 antibody [262C1a]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (adult ovary)
    Loading amount
    50 µg
    Specification
    adult ovary
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 29 2009

    Question


    With regard to ab58622 Mouse monoclonal antibody.
    I have discussed the issue with two of the senior pathologists here and our principle scientist.
    A couple of options we considered are 1: We could send you some slides with known FOX-L2 positive material to stain and return to us for evaluation.
    2: We acquire the antibody from you and evaluate it using our known positive material and send you details.
    This would include us billing you for the work done.
    We are not in a position to purchase the antibody under the terms offered in your last email.

    Read More
    Answer

    Thank you for your reply.
    We do not have immunohistochemical staining facilities available for testing customer-submitted samples.
    You could purchase the antibody and test it in IHC as part of our AbTrial program where you purchase the antibody at cost, submit your IHC data to us in the form of an Abreview (either positive or negative) and receive the price you paid for the antibody off your next purchase from Abcam in the form of a discount code.
    If you are interested in this offer, please let me know ant I will help get you set up. However, we do not provide testing services. I hope this information helps.

    Read More

    Question

    Can you put me in contact with anyone who is using ab58622 for immunohistochemistry on human tissue?

    Are there any histopathology laboratory’s using this FOX-L2 ?

    Read More
    Answer

    Thank you for contacting us and for your interest in our products.
    As far as we are aware, ab58622 has never been tested in IHC. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.
    If you would like to test the antibody in this untested application, please contact our Scientific Support team prior to the purchase by replying to this message as you may be eligible for our testing discount program.
    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
    To find out more about our Abreview system, please see the following link:
    https://www.abcam.com/abreviews
    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com