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AB309511

重组Anti-FOXK1/MNF抗体[EPR27320-65] - BSA and Azide free

Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • Advanced Validation
  • 了解详情

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Rabbit Recombinant Monoclonal FOXK1/MNF antibody. Carrier free. Suitable for ChIP-seq, WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

查看别名

MNF, FOXK1, Forkhead box protein K1, Myocyte nuclear factor

17 Images
Flow Cytometry (Intracellular) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling FOXK1/MNF with ab309510 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling FOXK1/MNF with ab309510 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing nuclear staining in K-562 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling FOXK1/MNF with ab309510 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon carcinoma (PMID : 27223064, PMID : 28623323). The section was incubated with ab309510 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXK1/MNF with ab309510 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon. The section was incubated with ab309510 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ChIP-sequencing - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using the same antibody clone in a different buffer formulation (ab309510).

Chromatin was prepared from HeLa cells. Cells were fixed with 1.5 mM EGS for 30 min, combined with 1% formaldehyde for 10 min. ChIP was performed with 107 cells and 8 µg of ab309510 [EPR27320-65]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

ChIP-sequencing - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using the same antibody clone in a different buffer formulation (ab309510).

Chromatin was prepared from HeLa cells. Cells were fixed with 1.5 mM EGS for 30 min, combined with 1% formaldehyde for 10 min. ChIP was performed with 107 cells and 8 µg of ab309510 [EPR27320-65]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

Immunoprecipitation - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IP

Supplier Data

Immunoprecipitation - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. FOXK1/MNF was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab309510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309510 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : ab309510 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309510 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 84 seconds Lysate was freshly made and used for Immunoprecipitation immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-FOXK1/MNF antibody [EPR27320-65] (<a href='/products/primary-antibodies/foxk1-mnf-antibody-epr27320-65-ab309510'>ab309510</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 84s

ChIP-sequencing - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using the same antibody clone in a different buffer formulation (ab309510).

Chromatin was prepared from HeLa cells. Cells were fixed with 1.5 mM EGS for 30 min, combined with 1% formaldehyde for 10 min. ChIP was performed with 107 cells and 8 µg of ab309510 [EPR27320-65]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling FOXK1/MNF with ab309510 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon. The section was incubated with ab309510 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling FOXK1/MNF with ab309510 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with ab309510 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling FOXK1/MNF with ab309510 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum.The section was incubated with ab309510 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling FOXK1/MNF with ab309510 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Flow Cytometry (Intracellular) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast) cells labelling FOXK1/MNF with ab309510 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • IP

Supplier Data

Immunoprecipitation - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. FOXK1/MNF was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab309510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309510 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab309510 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309510 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes Lysate was freshly made and used for Immunoprecipitation immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-FOXK1/MNF antibody [EPR27320-65] (<a href='/products/primary-antibodies/foxk1-mnf-antibody-epr27320-65-ab309510'>ab309510</a>) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3min

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • WB

Supplier Data

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 81 seconds

All lanes:

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] (<a href='/products/primary-antibodies/foxk1-mnf-antibody-epr27320-65-ab309510'>ab309510</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

K-562 transfected with siRNA specifically targeti FOXK1/MNF whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 95 kDa

false

Exposure time: 81s

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • WB

Supplier Data

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 147 seconds

All lanes:

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] (<a href='/products/primary-antibodies/foxk1-mnf-antibody-epr27320-65-ab309510'>ab309510</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 95 kDa

false

Exposure time: 147s

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)
  • WB

Supplier Data

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] - BSA and Azide free (AB309511)

This data was developed using ab309510, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 3 minutes

All lanes:

Western blot - Anti-FOXK1/MNF antibody [EPR27320-65] (<a href='/products/primary-antibodies/foxk1-mnf-antibody-epr27320-65-ab309510'>ab309510</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 95 kDa

false

Exposure time: 3min

不同偶联物与剂型 (2)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR27320-65

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

WB, ICC/IF, IHC-P, IP, ChIP-seq, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The FOXK1/MNF protein often called Forkhead Box K1 is a transcription factor that regulates gene expression linked to cell proliferation and differentiation. It has the molecular weight of approximately 84 kDa. Expressed in various tissues including muscle and brain FOXK1/MNF plays an essential role in regulating specific gene targets. The protein interacts with DNA via its forkhead domain influencing the transcriptional activity of numerous genes important for cellular functions.
Biological function summary

FOXK1/MNF is involved in the regulation of cell cycle progression and muscle regeneration. It forms part of a transcriptional complex that modulates gene expression to control these processes. In muscle tissue FOXK1/MNF influences the activation of satellite cells which are necessary for muscle repair and growth. This protein’s function in regulating cellular proliferation highlights its significance in tissue development and regeneration.

Pathways

The FOXK1/MNF protein is instrumental in the Wnt signaling and PI3K/AKT pathways. These pathways are significant for controlling cell proliferation differentiation and survival. Within these pathways FOXK1/MNF interacts with proteins such as beta-catenin in the Wnt signaling pathway. Through these interactions it modulates the transcription of genes responsible for various cellular functions further underlining its role in tissue regeneration and cancer development.

FOXK1/MNF links notably with certain cancers and muscular dystrophies. In cancer its overexpression can lead to unchecked cell proliferation and tumor growth interacting closely with proteins like PTEN a known tumor suppressor. In muscular dystrophy FOXK1/MNF may influence the disease progression by affecting the muscle regeneration process a vital element in maintaining muscle integrity. Understanding its expression and regulation can provide insights into the mechanisms driving these diseases and offer targets for potential therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed : 17670796). Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Involved in mTORC1-mediated metabolic reprogramming : in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism (By similarity). Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle : in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle (By similarity). Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population (By similarity). Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation (By similarity). Involved in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity). Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity). Represses myogenic differentiation by inhibiting MEFC activity (By similarity). Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus (PubMed : 25805136). Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression (PubMed : 25852164). Accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex; recruits the PR-DUB complex to specific FOXK1-bound genes (PubMed : 24634419, PubMed : 30664650).
See full target information FOXK1
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搭配使用产品

Sample Prep & Detection Kits

AB185913

High-Sensitivity ChIP Kit

ChIP - High-Sensitivity ChIP Kit (AB185913)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48

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