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Anti-FOXG1抗体[mAbcam 51774] (ab51774)

Submit a review Q&A (1)
Western blot - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
  • Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)

Key features and details

  • Mouse monoclonal [mAbcam 51774] to FOXG1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-FOXG1抗体[mAbcam 51774]
    参阅全部 FOXG1 一抗

  • 描述

    小鼠单克隆抗体[mAbcam 51774] to FOXG1

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat, Chicken, Cow, Zebrafish

  • 免疫原

    Synthetic peptide within Human FOXG1 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. Peptide available as ab19644.
    Database link: P55316
    (Peptide available as ab19644)

  • 阳性对照

    • This antibody gave a positive signal in human brain tissue lysate (ab29466).

  • 常规说明

    We can conjugate this antibody to FITC for you (please see ab150200 for details).

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51774于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
说明
Flow Cyt (Intra)
Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能

    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

  • 组织特异性

    Expression is restricted to the neurons of the developing telencephalon.

  • 疾病相关

    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.

  • 序列相似性

    Contains 1 fork-head DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • BF-1 antibody
      • BF-2 antibody
      • BF1 antibody
      • BF2 antibody
      • Brain factor 1 antibody
      • Brain factor 2 antibody
      • FHKL antibody
      • FKH2 antibody
      • FKHL1 antibody
      • FKHL2 antibody
      • FKHL3 antibody
      • FKHL4 antibody
      • Forkhead box protein G1 antibody
      • Forkhead box protein G1A antibody
      • Forkhead box protein G1B antibody
      • Forkhead box protein G1C antibody
      • Forkhead like 1 antibody
      • Forkhead like 2 antibody
      • Forkhead like 3 antibody
      • Forkhead like 4 antibody
      • Forkhead-related protein FKHL1 antibody
      • Forkhead-related protein FKHL2 antibody
      • Forkhead-related protein FKHL3 antibody
      • FOXG1 antibody
      • FOXG1_HUMAN antibody
      • FOXG1A antibody
      • FOXG1B antibody
      • FOXG1C antibody
      • HBF 1 antibody
      • HBF G2 antibody
      • hBF-2 antibody
      • HBF2 antibody
      • HFK1 antibody
      • HFK2 antibody
      • HFK3 antibody
      • KHL2 antibody
      • Oncogene QIN antibody
      • QIN antibody
      see all

    图片

    • Western blot - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
      Western blot - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
      Anti-FOXG1 antibody [mAbcam 51774] (ab51774) at 5 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

      Secondary
      Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution

      Performed under reducing conditions.

      Predicted band size: 50 kDa
      Observed band size: 50 kDa
      Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
      Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [mAbcam 51774] (ab51774)
      Overlay histogram showing SHSY-5Y cells stained with ab51774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51774, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献 (0)

    发表研究结果有使用 ab51774？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab51774 尚未被引用在任何文献中。

    客户评价及客户问答

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    提交评价 提交问题

    Question

    Hello,
    I would like know if the antibodie Anti-FOXG1 [antibody [mAbcam 51774] (ab51774)] is reactive with chicken, and if the antibodie can be used to immunofluorescence staining in parafin.
    Thanks.

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately ab51774 has not been tested either with Chicken, nor IHC-P. It doesn’t mean it won’t work in this species / application, but we don’t have data to support it.

    However, due to sequence homology, the antibody is very likely to cross react with Chicken. If you are interested in participating in our antibodies evaluation system (Abreview), and test ab51774 with IH –P and / or chicken species (by WB or Flow Cytometry) I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

    Therefore, I can offer a discount off a future purchase if you buy an antibody and test it in a new species or with a new application and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab51774 in IHC-P and / or chicken species. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

    3. Test it in the new species / application.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: http://ops.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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