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Neuroscience Neurology process Neural Signal Transduction

Anti-FOXG1抗体(ab3394)

  • Datasheet
Reviews (1)Q&A (3)References (3)

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Western blot - Anti-FOXG1 antibody (ab3394)

    Key features and details

    • Goat polyclonal to FOXG1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-FOXG1 antibody [EPR18987] (ab196868)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-FOXG1抗体
      参阅全部 FOXG1 一抗
    • 描述

      山羊多克隆抗体to FOXG1
    • 宿主

      Goat
    • 特异性

      This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Rat
    • 免疫原

      Synthetic peptide corresponding to Human FOXG1 aa 457-469 (C terminal).
      Sequence:

      C-HQNQGSSSNPLIH


      Database link: P55316
      (Peptide available as ab22945)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • FOXG

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3394于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa).

    A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.

    说明
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa).

    A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.

    靶标

    • 功能

      Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
    • 组织特异性

      Expression is restricted to the neurons of the developing telencephalon.
    • 疾病相关

      Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
    • 序列相似性

      Contains 1 fork-head DNA-binding domain.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession P55316 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 2290 Human
      • Entrez Gene: 24370 Rat
      • Omim: 164874 Human
      • SwissProt: P55316 Human
      • SwissProt: Q00939 Rat
      • Unigene: 695962 Human
      • Unigene: 708841 Human
      • Unigene: 9864 Rat
      • 别名

        • BF-1 antibody
        • BF-2 antibody
        • BF1 antibody
        • BF2 antibody
        • Brain factor 1 antibody
        • Brain factor 2 antibody
        • FHKL antibody
        • FKH2 antibody
        • FKHL1 antibody
        • FKHL2 antibody
        • FKHL3 antibody
        • FKHL4 antibody
        • Forkhead box protein G1 antibody
        • Forkhead box protein G1A antibody
        • Forkhead box protein G1B antibody
        • Forkhead box protein G1C antibody
        • Forkhead like 1 antibody
        • Forkhead like 2 antibody
        • Forkhead like 3 antibody
        • Forkhead like 4 antibody
        • Forkhead-related protein FKHL1 antibody
        • Forkhead-related protein FKHL2 antibody
        • Forkhead-related protein FKHL3 antibody
        • FOXG1 antibody
        • FOXG1_HUMAN antibody
        • FOXG1A antibody
        • FOXG1B antibody
        • FOXG1C antibody
        • HBF 1 antibody
        • HBF G2 antibody
        • hBF-2 antibody
        • HBF2 antibody
        • HFK1 antibody
        • HFK2 antibody
        • HFK3 antibody
        • KHL2 antibody
        • Oncogene QIN antibody
        • QIN antibody
        see all

      图片

      • Western blot - Anti-FOXG1 antibody (ab3394)
        Western blot - Anti-FOXG1 antibody (ab3394)
        ab3394 staining (2µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab3394 (0.3 µg/ml) staining of Human Brain lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

      实验方案

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (3)

      发表研究结果有使用 ab3394?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3394 被引用在 3 文献中.

      • Schäffner I  et al. Differential vulnerability of adult neurogenic niches to dosage of the neurodevelopmental-disorder linked gene Foxg1. Mol Psychiatry 28:497-514 (2023). PubMed: 35318461
      • Li JV  et al. Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells. Mol Endocrinol 27:1113-27 (2013). PubMed: 23660594
      • Regad T  et al. The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF. Nat Cell Biol 9:531-40 (2007). ICC/IF ; Mouse . PubMed: 17435750

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-FOXG1 antibody

      Poor
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (brain)
      Specification
      brain
      Fixative
      Paraformaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 11 2005

      Question

      The replacement we received for ab3394, ab18259, also fails to detect FOXG1 in human neural progenitor cell cytochemistry.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 06 2011

      Answer

      I have issued a free of charge replacement for ab18259, with the order number *****. The replacement is ab3394. To check the status of the order please contact our Customer Service team and reference this number. I suggested in my conversation with your colleague that an alternative to PFA fixation may give a better result this time: ice-cold acetone or methanol, or a 50:50 mixture of the two, for 10-20 minutes, followed by rinsing in buffer and blocking. No permeabilization is necessary. If you have any questions, please contact me.

      Read More

      Abcam Scientific Support

      回复于 Oct 06 2011

      Question

      No staining of human neuronal progenitor cells.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 28 2011

      Answer

      Thanks for contacting us and letting us know about the problems with ab3394. I am sending a free of charge vial of ab18259 on the order ***, which should arrive shortly. Please keep me updated on the results using this replacement, and let me know if you need anything further.

      Read More

      Abcam Scientific Support

      回复于 Sep 28 2011

      Question

      I would like to use BF-1 (Foxg1) antibody on human ES cells-derived neurons (immunocytochemistry). Your web site says the foxg1 AB is ok for Western blot. Any info on ICH? May i have a small aliq. to test this AB on my slides?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 16 2004

      Answer

      Thank you very much for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

      Read More

      Abcam Scientific Support

      回复于 Apr 21 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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