Anti-FOXC2抗体(ab5060)
Key features and details
- Goat polyclonal to FOXC2
- Suitable for: WB, ChIP
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-FOXC2抗体
参阅全部 FOXC2 一抗 -
描述
山羊多克隆抗体to FOXC2 -
宿主
Goat -
经测试应用
适用于: WB, ChIPmore details
不适用于: IHC-Fr -
种属反应性
与反应: Mouse, Human, African green monkey
预测可用于: Rat, Cow, Dog -
免疫原
Synthetic peptide:
RHAAPYSYDCTKY
, corresponding to C terminal amino acids 489-501 of Human FOXC2. -
阳性对照
- WB: HEK-293 nuclear lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 60-70 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product. |
|
ChIP |
Use at an assay dependent concentration. PubMed: 18579532
|
说明 |
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WB
Use a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 60-70 kDa (predicted molecular weight: 53 kDa). A 1 hour primary incubation is recommended for this product. |
ChIP
Use at an assay dependent concentration. PubMed: 18579532 |
靶标
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功能
Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. -
疾病相关
Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices). -
序列相似性
Contains 1 fork-head DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2303 Human
- Entrez Gene: 14234 Mouse
- Entrez Gene: 171356 Rat
- Omim: 602402 Human
- SwissProt: Q99958 Human
- SwissProt: Q61850 Mouse
- SwissProt: Q63246 Rat
- Unigene: 436448 Human
see all -
别名
- Drosphilia Forkhead Homolog Like 14 antibody
- FKHL 14 antibody
- FKHL14 antibody
see all
图片
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Lane 1 : Anti-FOXC2 antibody (ab5060) at 2 µg/ml
Lane 2 : Anti-FOXC2 antibody (ab5060) at 1 µg/ml
Lane 1 : nuclear HEK293 lysate in RIPA buffer
Lane 2 : human pancreas whole cell lysate (negative control) in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60-70 kDa why is the actual band size different from the predicted?Primary incubation: 1 hour at room temperature.
Negative Control: Human Pancreas lysate.
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Western blot - Anti-FOXC2 antibody (ab5060)Image courtesy of a review by Michal Bell submitted 19 August 2004
FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector.
数据表及文件
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Datasheet download
文献 (27)
ab5060 被引用在 27 文献中.
- Lin F et al. Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer. J Exp Clin Cancer Res 41:129 (2022). PubMed: 35392966
- Liu H et al. The FENDRR/FOXC2 Axis Contributes to Multidrug Resistance in Gastric Cancer and Correlates With Poor Prognosis. Front Oncol 11:634579 (2021). PubMed: 33869020
- Norden PR et al. Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation. Elife 9:N/A (2020). PubMed: 32510325
- Tavian D et al. FOXC2 Disease Mutations Identified in Lymphedema Distichiasis Patients Impair Transcriptional Activity and Cell Proliferation. Int J Mol Sci 21:N/A (2020). PubMed: 32698337
- Zhang Y et al. Activation of mitogen-activated protein kinases in satellite glial cells of the trigeminal ganglion contributes to substance P-mediated inflammatory pain. Int J Oral Sci 11:24 (2019). PubMed: 31501412