重组Anti-FOXC1抗体[EPR20678] (ab223850)
![Western blot - Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-297266-anti-foxc1-antibody-epr20678-western-blot.jpg "Western blot - Anti-FOXC1 antibody [EPR20678] (ab223850)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20678] to FOXC1
- Suitable for: Flow Cyt (Intra), IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXC1抗体[EPR20678]
参阅全部 FOXC1 一抗 -
描述
兔单克隆抗体[EPR20678] to FOXC1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IP, WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK-293T, HeLa, 4T1, MDA-MB-231, MDA-MB-435S, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen lysate. IHC-P: Human gastric cancer, pancreatic cancer and basal-like breast cancer tissues. Flow Cyt (intra): HEK-293T cells. IP: HeLa whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20678 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223850于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| IP |
1/30.
|
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| WB |
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC not suitable for mouse & rat tissues. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. |
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC not suitable for mouse & rat tissues. |
靶标
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功能
Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. -
组织特异性
Expressed in all tissues and cell lines examined. -
疾病相关
Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3) [MIM:602482]; also known as Axenfeld-Rieger syndrome (ARS) or Axenfeld syndrome or Axenfeld anomaly. It is characterized by posterior corneal embryotoxon, prominent Schwalbe line and iris adhesion to the Schwalbe line. Other features may be hypertelorism (wide spacing of the eyes), hypoplasia of the malar bones, congenital absence of some teeth and mental retardation. When associated with tooth anomalies, the disorder is known as Rieger syndrome. Glaucoma is a progressive blinding condition that occurs in approximately half of patients with Axenfeld-Rieger malformations.
Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA) [MIM:601631]. IGDA is an autosomal dominant phenotype characterized by iris hypoplasia, goniodysgenesis, and juvenile glaucoma.
Defects in FOXC1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2296 Human
- Entrez Gene: 17300 Mouse
- Entrez Gene: 364706 Rat
- GenBank: NP_001444 Human
- Omim: 601090 Human
- SwissProt: Q12948 Human
- SwissProt: Q61572 Mouse
- Unigene: 348883 Human
see all -
别名
- ARA antibody
- FKH L7 antibody
- FKHL 7 antibody
see all
图片
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Western blot - Anti-FOXC1 antibody [EPR20678] (ab223850)All lanes : Anti-FOXC1 antibody [EPR20678] (ab223850) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : 4T1 (mouse mammary gland carcinoma epithelial cell line) whole cell lysate at 10 µg
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate at 20 µg
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Lane 8 : Human fetal spleen lysate at 10 µg
Secondary
Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated)
Lane 8 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution (Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated)
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 15 seconds; Lanes 2-3 & 6-8: 3 minutes; Lanes 4-5: 1 minute.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass is consistent with what has been described in the literature (PMID: 27708239).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-297267-anti-foxc1-antibody-epr20678-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human gastric cancer is observed(PMID:24329718). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-297268-anti-foxc1-antibody-epr20678-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human pancreatic cancer is observed(PMID:23242609). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-297269-anti-foxc1-antibody-epr20678-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)Immunohistochemical analysis of paraffin-embedded human basal-like breast cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human basal-like breast cancer (PMID:27708239; PMID: 20406990). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-297272-anti-foxc1-antibody-epr20678-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-FOXC1 antibody [EPR20678] (ab223850)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling FOXC1 with ab223850 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-297273-anti-foxc1-antibody-epr20678-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] (ab223850)FOXC1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223850 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223850 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab223850 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223850 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The molecular mass is consistent with what has been described in the literature (PMID: 22493429).
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![Anti-FOXC1 antibody [EPR20678] (ab223850)](https://www.abcam.cn/ps/products/223/ab223850/Images/ab223850-9-benefits-of-recombinant-antibodies.png)
Anti-FOXC1 antibody [EPR20678] (ab223850)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab223850 被引用在 4 文献中.
- Wu JH et al. Exosome-Mediated miR-4792 Transfer Promotes Bladder Cancer Cell Proliferation via Enhanced FOXC1/c-Myc Signaling and Warburg Effect. J Oncol 2022:5680353 (2022). PubMed: 35096062
- Zhou Y et al. The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways. Stem Cell Res Ther 12:64 (2021). PubMed: 33461605
- Huang H et al. Defining super-enhancer landscape in triple-negative breast cancer by multiomic profiling. Nat Commun 12:2242 (2021). PubMed: 33854062
- Wang J et al. Identification of a novel microRNA-141-3p/Forkhead box C1/ß-catenin axis associated with rheumatoid arthritis synovial fibroblast function in vivo and in vitro. Theranostics 10:5412-5434 (2020). PubMed: 32373221