重组Anti-FOXA2抗体[EPR4466] (ab108422)
![Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-242449-anti-foxa2-antibody-epr4466-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4466] to FOXA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-FOXA2抗体[EPR4466]
参阅全部 FOXA2 一抗 -
描述
兔单克隆抗体[EPR4466] to FOXA2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human colon cancer, fetal colon and mouse lung tissue lysates and HepG2 whole cell lysate (ab7900). IHC-P: Human hepatocellular carcinoma and mouse liver tissue; Human colon tissue; Mouse brain (substantia nigra) tissue. ICC/IF: HT-29 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4466 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-FOXA2 antibody [EPR4466] (ab193864)
- Alexa Fluor® 647 Anti-FOXA2 antibody [EPR4466] (ab193879)
- HRP Anti-FOXA2 antibody [EPR4466] (ab193880)
- Anti-FOXA2 antibody [EPR4466] - BSA and Azide free (ab220810)
- Alexa Fluor® 568 Anti-FOXA2 antibody [EPR4466] (ab313032)
- Alexa Fluor® 750 Anti-FOXA2 antibody [EPR4466] (ab320902)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108422于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 52 kDa.
For unpurified use at 1/1000 - 1/10000. |
|
| IHC-P | (6) |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
| ICC/IF |
1/300.
For unpurified use at 1/250 - 1/500. |
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 52 kDa. For unpurified use at 1/1000 - 1/10000. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
|
ICC/IF
1/300. For unpurified use at 1/250 - 1/500. |
靶标
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功能
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3170 Human
- Entrez Gene: 15376 Mouse
- Entrez Gene: 25099 Rat
- Omim: 600288 Human
- SwissProt: Q9Y261 Human
- SwissProt: P35583 Mouse
- SwissProt: P32182 Rat
- Unigene: 155651 Human
see all -
别名
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4466] (ab108422)
Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling FOXA2 with purified ab108422 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
-ve control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
-ve control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-242447-anti-foxa2-antibody-epr4466-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-121142-anti-foxa2-antibody-epr4466-western-blot.jpg)
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)All lanes : Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal colon tissue lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-242448-anti-foxa2-antibody-epr4466-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling FOXA2 with purified ab108422 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-242445-anti-foxa2-antibody-epr4466-western-blot.jpg)
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/10000 dilution (purified) + Human colon cancer tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-267986-anti-foxa2-antibody-epr4466-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human colon sections labelling FOXA2 with ab108422 at dilution of 1/500. The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.
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![Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-242446-anti-foxa2-antibody-epr4466-western-blot.jpg)
Western blot - Anti-FOXA2 antibody [EPR4466] (ab108422)Anti-FOXA2 antibody [EPR4466] (ab108422) at 1/2000 dilution (purified) + Mouse lung tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-121141-anti-foxa2-antibody-epr4466-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FOXA2 with unpurified ab108422 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-264049-anti-foxa2-antibody-epr4466-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (ab108422)This image is courtesy of Carl Hobbs, King's College London, United Kingdomab108422 staining of FOXA2 in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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![Anti-FOXA2 antibody [EPR4466] (ab108422)](https://www.abcam.cn/ps/products/108/ab108422/Images/ab108422-12-benefits-of-recombinant-antibodies.png)
Anti-FOXA2 antibody [EPR4466] (ab108422)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (104)
ab108422 被引用在 104 文献中.
- Harding J et al. Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts. Nat Biomed Eng 8:427-442 (2024). PubMed: 37996616
- Ma X et al. The Uterine Melatonergic Systems of AANAT and Melatonin Membrane Receptor 2 (MT2) Are Essential for Endometrial Receptivity and Early Implantation in Mice. Int J Mol Sci 24:N/A (2023). PubMed: 37108290
- Feng J et al. EBNA2 mediates lipid metabolism and tumorigenesis through activation of ATF4 pathway. Am J Cancer Res 13:1363-1376 (2023). PubMed: 37168348
- Zhang H et al. Dietary disodium fumarate supplementation alleviates subacute ruminal acidosis (SARA)-induced liver damage by inhibiting pyroptosis via mitophagy-NLRP3 inflammasome pathway in lactating Hu sheep. Front Immunol 14:1197133 (2023). PubMed: 37275885
- Rizo JA et al. Estrogen receptor alpha regulates uterine epithelial lineage specification and homeostasis. iScience 26:107568 (2023). PubMed: 37622003