重组Anti-Folate Binding蛋白/FBP抗体[EPR23387-276] - BSA and Azide free (ab273159)
![Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)](https://www.abcam.cn/ps/products/273/ab273159/Images/ab273159-1-anti-folate-binding-proteinfbp-antibody-epr23387276-immunocytochemistry-jar.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23387-276] to Folate Binding Protein/FBP - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Folate Binding蛋白/FBP抗体[EPR23387-276] - BSA and Azide free
参阅全部 Folate Binding Protein/FBP 一抗 -
描述
兔单克隆抗体[EPR23387-276] to Folate Binding蛋白/FBP - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cytmore details
不适用于: IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: JAR cells. Flow Cyt: JAR cells.
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常规说明
ab273159 is the carrier-free version of ab272715.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23387-276 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
组织特异性
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
疾病相关
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
序列相似性
Belongs to the folate receptor family. -
翻译后修饰
Eight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
细胞定位
Cell membrane. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2348 Human
- Omim: 136430 Human
- SwissProt: P15328 Human
- Unigene: 73769 Human
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别名
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized JAR cells labelling Folate Binding Protein/FBP with ab272715 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in JAR cell line. Negative contro:l K562 PMID: 15899836. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272715).
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![Flow Cytometry - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)](https://www.abcam.cn/ps/products/273/ab273159/Images/ab273159-2-anti-folate-binding-proteinfbp-antibody-epr23387276-flow-cytometry-k562.jpg)
Flow Cytometry - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)Flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) / JAR (Human placenta choriocarcinoma epithelial cell, Right) cells labelling Folate Binding Protein/FBP with ab272715 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2000 dilution was used as the secondary antibody. Negative control: K-562 (PMID: 15899836). Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272715).
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![Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)](https://www.abcam.cn/ps/products/273/ab273159/Images/ab273159-3-benefits-of-recombinant-antibodies.png)
Anti-Folate Binding Protein/FBP antibody [EPR23387-276] - BSA and Azide free (ab273159)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273159 尚未被引用在任何文献中。
