重组Anti-Folate Binding蛋白/FBP抗体[EPR23387-276] (ab272715)
![Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)](https://www.abcam.cn/ps/products/272/ab272715/Images/ab272715-1-anti-folate-binding-proteinfbp-antibody-epr23387276-immunocytochemistry-jar.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23387-276] to Folate Binding Protein/FBP
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Folate Binding蛋白/FBP抗体[EPR23387-276]
参阅全部 Folate Binding Protein/FBP 一抗 -
描述
兔单克隆抗体[EPR23387-276] to Folate Binding蛋白/FBP -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cytmore details
不适用于: IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: JAR cells. Flow Cyt: JAR cells..
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23387-276 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272715于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50.
|
|
| Flow Cyt |
1/500.
|
| 说明 |
|---|
|
ICC/IF
1/50. |
|
Flow Cyt
1/500. |
靶标
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功能
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
组织特异性
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
疾病相关
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
序列相似性
Belongs to the folate receptor family. -
翻译后修饰
Eight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
细胞定位
Cell membrane. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2348 Human
- Omim: 136430 Human
- SwissProt: P15328 Human
- Unigene: 73769 Human
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别名
- adult antibody
- Adult folate binding protein antibody
- Adult folate-binding protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized JAR cells labelling Folate Binding Protein/FBP with ab272715 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in JAR cell line. Negative contro:l K562 PMID: 15899836. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
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![Flow Cytometry - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)](https://www.abcam.cn/ps/products/272/ab272715/Images/ab272715-2-anti-folate-binding-proteinfbp-antibody-epr23387276-flow-cytometry-k562.jpg)
Flow Cytometry - Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)Flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) / JAR (Human placenta choriocarcinoma epithelial cell, Right) cells labelling Folate Binding Protein/FBP with ab272715 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2000 dilution was used as the secondary antibody. Negative control: K-562 (PMID: 15899836). Gated on viable cells.
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![Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)](https://www.abcam.cn/ps/products/272/ab272715/Images/ab272715-3-benefits-of-recombinant-antibodies.png)
Anti-Folate Binding Protein/FBP antibody [EPR23387-276] (ab272715)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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