Anti-FNDC5抗体(ab131390)
Key features and details
- Rabbit polyclonal to FNDC5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FNDC5抗体
参阅全部 FNDC5 一抗 -
描述
兔多克隆抗体to FNDC5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Macaque monkey, Orangutan
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免疫原
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: K562; C2C12.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131390于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa). |
靶标
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序列相似性
Contains 1 fibronectin type-III domain. -
细胞定位
Peroxisome membrane. Imported in peroxisomes through the PEX5 receptor pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 252995 Human
- Entrez Gene: 384061 Mouse
- Entrez Gene: 260327 Rat
- Entrez Gene: 555635 Zebrafish
- Omim: 611906 Human
- SwissProt: Q8NAU1 Human
- SwissProt: Q8K4Z2 Mouse
- SwissProt: Q8K3V5 Rat
see all -
别名
- Fibronectin type III domain containing 5 antibody
- Fibronectin type III domain-containing protein 5 antibody
- Fibronectin type III repeat containing protein 2 antibody
see all
图片
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Western blot - Anti-FNDC5 antibody (ab131390)All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-FNDC5 antibody (ab131390)All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab131390 被引用在 21 文献中.
- Song D et al. Low-intensity pulsed ultrasound triggers a beneficial neuromodulation in dementia mice with chronic cerebral hypoperfusion via activation of hippocampal Fndc5/irisin signaling. J Transl Med 21:139 (2023). PubMed: 36823656
- Wu Y et al. Irisin attenuates angiotensin II-induced atrial fibrillation and atrial fibrosis via LOXL2 and TGFβ1/Smad2/3 signaling pathways. Iran J Basic Med Sci 26:717-724 (2023). PubMed: 37275755
- Lee H et al. Annona muricate Extract Supplementation Contributes to Improve Aberrant Multi-Organ Energy Metabolism via Muscle-Brain Connectivity in Diabetic Mice. Nutrients 15:N/A (2023). PubMed: 37299522
- Baranowski RW et al. Toward countering muscle and bone loss with spaceflight: GSK3 as a potential target. iScience 26:107047 (2023). PubMed: 37360691
- Hou YC et al. Indoxyl sulfate induced frailty in patients with end-stage renal disease by disrupting the PGC-1α-FNDC5 axis. Aging (Albany NY) 15:11532-11545 (2023). PubMed: 37878003