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AB131390

Anti-FNDC5抗体

Anti-FNDC5 antibody

2

(1 Review)

|

(22 Publications)

Rabbit Polyclonal FNDC5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 22 publications.

查看别名

FRCP2, FNDC5, Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2

2 Images
Western blot - Anti-FNDC5 antibody (AB131390)
  • WB

Ap

Western blot - Anti-FNDC5 antibody (AB131390)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-FNDC5 antibody (ab131390) at 1 µg/mL

Lane 1:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

C2C12 (Mouse myoblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Exposure time: 30s

Western blot - Anti-FNDC5 antibody (AB131390)
  • WB

Lab

Western blot - Anti-FNDC5 antibody (AB131390)

All lanes:

Western blot - Anti-FNDC5 antibody (ab131390) at 1 µg/mL

Lane 1:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

C2C12 (Mouse myoblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa,45 kDa,50 kDa

true

Exposure time: 20min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Irisin. Mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.
See full target information FNDC5

文献 (22)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e30984 PubMed38803993

2024

Astragaloside IV inhibits angiotensin II-induced atrial fibrosis and atrial fibrillation by SIRT1/PGC-1α/FNDC5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinpeng Cong,Xi Zhu,Xiaogang Zhang,Zhongping Ning

Aging 15:11532-11545 PubMed37878003

2023

Indoxyl sulfate induced frailty in patients with end-stage renal disease by disrupting the PGC-1α-FNDC5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chou Hou,Min-Tser Liao,Kuo-Wang Tsai,Cai-Mei Zheng,Hui-Wen Chiu,Kuo-Cheng Lu

iScience 26:107047 PubMed37360691

2023

Toward countering muscle and bone loss with spaceflight: GSK3 as a potential target.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan W Baranowski,Jessica L Braun,Briana L Hockey,Jenalyn L Yumol,Mia S Geromella,Colton J F Watson,Nigel Kurgan,Holt N Messner,Kennedy C Whitley,Adam J MacNeil,Guillemette Gauquelin-Koch,Fabrice Bertile,William Gittings,Rene Vandenboom,Wendy E Ward,Val A Fajardo

Iranian journal of basic medical sciences 26:717-724 PubMed37275755

2023

Irisin attenuates angiotensin II-induced atrial fibrillation and atrial fibrosis via LOXL2 and TGFβ1/Smad2/3 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yingbiao Wu,Jun Luo,Xiang Song,Wei Gu,Saihua Wang,Shuwen Hao,Zhiwu Dong,Zhongping Ning

Nutrients 15: PubMed37299522

2023

Extract Supplementation Contributes to Improve Aberrant Multi-Organ Energy Metabolism via Muscle-Brain Connectivity in Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Heaji Lee,Sun Yeou Kim,Yunsook Lim

Journal of translational medicine 21:139 PubMed36823656

2023

Low-intensity pulsed ultrasound triggers a beneficial neuromodulation in dementia mice with chronic cerebral hypoperfusion via activation of hippocampal Fndc5/irisin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Degang Song,Xiaomin Chen,Na Zhou,Yi Yuan,Shuo Geng,Cong Zhang,Zhe Zhao,Xingran Wang,Xinran Bao,Xifa Lan,Xiangjian Zhang

Diabetes 71:2777-2792 PubMed35802043

2022

Exercise-Linked Skeletal Irisin Ameliorates Diabetes-Associated Osteoporosis by Inhibiting the Oxidative Damage-Dependent miR-150-FNDC5/Pyroptosis Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jyotirmaya Behera,Jessica Ison,Michael J Voor,Neetu Tyagi

Pharmaceutical biology 60:1106-1115 PubMed35639355

2022

Myostain is involved in ginsenoside Rb1-mediated anti-obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Shi Li,Jiang-Ying Kuang,Gui-Jun Liu,Wei-Jie Wu,Xian-Lun Yin,Hao-Dong Li,Lei Wang,Tao Qin,Wen-Cheng Zhang,Yuan-Yuan Sun

Pharmaceutics 14: PubMed35745732

2022

Pentamethylquercetin Regulates Lipid Metabolism by Modulating Skeletal Muscle-Adipose Tissue Crosstalk in Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhao Wu,Jingxia Du,Zhi Li,Wei He,Min Wang,Manwen Jin,Lei Yang,Hui Liu

Nutrients 13: PubMed34206655

2021

Anti-Atherogenic Effect of 10% Supplementation of Anchovy () Waste Protein Hydrolysates in ApoE-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Maria Abbate,Francesco Macrì,Francesca Arfuso,Carmelo Iaria,Fabiano Capparucci,Carmelo Anfuso,Antonio Ieni,Luca Cicero,Giovanni Briguglio,Giovanni Lanteri
View all publications

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