Anti-FMRP (phospho S499)抗体(ab183319)
Key features and details
- Rabbit polyclonal to FMRP (phospho S499)
- Suitable for: ELISA, WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-FMRP (phospho S499)抗体
参阅全部 FMRP 一抗 -
描述
兔多克隆抗体to FMRP (phospho S499) -
宿主
Rabbit -
特异性
ab183319 is specific for FMRP when phosphorylated at serine 499. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase. -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Sheep, Chicken, Cow, Dog, Human, Xenopus laevis, Non human primates, Zebrafish -
免疫原
Synthetic peptide corresponding to Rat FMRP (phospho S499).
Database link: Q80WE1 -
阳性对照
- Rat hippocampal lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab183319 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183319于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 67 kDa.
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说明 |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 67 kDa. |
靶标
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功能
Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). -
组织特异性
Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. -
疾病相关
Defects in FMR1 are the cause of fragile X syndrome (FRAX) [MIM:300624]. Fragile X syndrome is a common genetic disease (has a prevalence of one in every 2000 children) which is characterized by moderate to severe mental retardation, macroorchidism (enlargement of the testicles), large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Defects in FMR1 are the cause of fragile X tremor/ataxia syndrome (FXTAS) [MIM:300623]. In FXTAS, the expanded repeats range in size from 55 to 200 repeats and are referred to as 'premutations'. Full repeat expansions with greater than 200 repeats results in fragile X mental retardation syndrome [MIM:300624]. Carriers of the premutation typically do not show the full fragile X syndrome phenotype, but comprise a subgroup that may have some physical features of fragile X syndrome or mild cognitive and emotional problems.
Defects in FMR1 are the cause of premature ovarian failure syndrome type 1 (POF1) [MIM:311360]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
序列相似性
Belongs to the FMR1 family.
Contains 2 KH domains. -
翻译后修饰
Phosphorylated on several serine residues. -
细胞定位
Cytoplasm. Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 2332 Human
- Entrez Gene: 14265 Mouse
- Entrez Gene: 24948 Rat
- Entrez Gene: 259191 Zebrafish
- Omim: 309550 Human
- SwissProt: Q06787 Human
- SwissProt: P35922 Mouse
- SwissProt: Q80WE1 Rat
see all -
别名
- FMR 1 antibody
- Fmr1 antibody
- Fmr1 gene antibody
see all
图片
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All lanes : Anti-FMRP (phospho S499) antibody (ab183319) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate treated with Lambda Phosphatase (400 units / 100 µL lysate for 30 minutes)
Predicted band size: 67 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab183319 被引用在 3 文献中.
- Borland JM et al. Aggression Results in the Phosphorylation of ERK1/2 in the Nucleus Accumbens and the Dephosphorylation of mTOR in the Medial Prefrontal Cortex in Female Syrian Hamsters. Int J Mol Sci 24:N/A (2023). PubMed: 36674893
- D'Souza MN et al. Function of FMRP Domains in Regulating Distinct Roles of Neuronal Protein Synthesis. Mol Neurobiol 59:7370-7392 (2022). PubMed: 36181660
- Kute PM et al. NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP. Mol Brain 12:65 (2019). PubMed: 31291981