重组Anti-Flt3 ligand/Flt3L抗体[EP1140Y] (ab52648)
![Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.cn/ps/products/52/ab52648/Images/ab52648-298502-anti-flt3-ligand-flt3l-antibody-ep1140y-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1140Y] to Flt3 ligand/Flt3L
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Flt3 ligand/Flt3L抗体[EP1140Y]
参阅全部 Flt3 ligand/Flt3L 一抗 -
描述
兔单克隆抗体[EP1140Y] to Flt3 ligand/Flt3L -
宿主
Rabbit -
特异性
The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
-
经测试应用
适用于: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rhesus monkey
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-P: Human liver. WB: recombinant protein; ICC/IF: MCF7 cells; Flow Cyt (intra): Hela cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1140Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52648于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
|
| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
|
|
| IHC-P | (3) |
1/180. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/100 - 1/250. |
|
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa). |
|
IHC-P
1/180. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2323 Human
- Entrez Gene: 719239 Rhesus monkey
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
-
别名
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with Purified ab52648 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
![Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.cn/ps/products/52/ab52648/Images/ab52648-299546-anti-flt3-ligand-flt3l-antibody-ep1140y-western-blot.jpg)
Western blot - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648) at 1/100000 dilution (purified) + Human Flt3 ligand/Flt3L (aa 1 to 235) recombinant protein, 15ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
![Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.cn/ps/products/52/ab52648/Images/ab52648-298517-anti-flt3-ligand-flt3l-antibody-ep1140y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Flt3 ligand/Flt3L with ab52648 at 1/20 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) / (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.cn/ps/products/52/ab52648/Images/ab52648-298373-anti-flt3-ligand-flt3l-antibody-ep1140y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Flt3 ligand with purified ab52648 at 1:180 dilution (2.9 μg/ml). ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)](https://www.abcam.cn/ps/products/52/ab52648/Images/ab52648-10-benefits-of-recombinant-antibodies.png)
Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (ab52648)
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab52648 被引用在 7 文献中.
- Zhang Z et al. Combined treatment of marizomib and cisplatin modulates cervical cancer growth and invasion and enhances antitumor potential in vitro and in vivo. Front Oncol 12:974573 (2022). PubMed: 36110967
- Li H et al. MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis. Front Oncol 11:649980 (2021). PubMed: 34178637
- Joshi SK et al. The AML microenvironment catalyzes a stepwise evolution to gilteritinib resistance. Cancer Cell 39:999-1014.e8 (2021). PubMed: 34171263
- Yuan X et al. Mesenchymal stem cell therapy induces FLT3L and CD1c+ dendritic cells in systemic lupus erythematosus patients. Nat Commun 10:2498 (2019). PubMed: 31175312
- Traer E et al. FGF2 from Marrow Microenvironment Promotes Resistance to FLT3 Inhibitors in Acute Myeloid Leukemia. Cancer Res 76:6471-6482 (2016). PubMed: 27671675
- Taylor RM et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). PubMed: 22906984
- Tobón GJ et al. The Fms-like tyrosine kinase 3 ligand, a mediator of B cell survival, is also a marker of lymphoma in primary Sjögren's syndrome. Arthritis Rheum 62:3447-56 (2010). IF ; Human . PubMed: 20556820