Anti-Flt3 ligand/Flt3L抗体(ab9688)
Key features and details
- Rabbit polyclonal to Flt3 ligand/Flt3L
- Suitable for: Flow Cyt, WB, Neutralising, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Flt3 ligand/Flt3L抗体
参阅全部 Flt3 ligand/Flt3L 一抗 -
描述
兔多克隆抗体to Flt3 ligand/Flt3L -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, WB, Neutralising, Sandwich ELISAmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human Flt3 ligand/Flt3L aa 1-200. Highly pure>98%. Produced in E. coli.
Database link: P49771 -
常规说明
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration. PubMed: 22897934
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
|
| Neutralising |
Use at an assay dependent concentration.
Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) |
|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
In conjunction with ab84261 rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. PubMed: 22897934 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. The detection limit for recombinant Flt3 ligand/Flt3L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Neutralising
Use at an assay dependent concentration. Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml) |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. In conjunction with ab84261 rabbit polyclonal to Flt3 ligand/Flt3L (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Flt3 ligand/Flt3L. |
靶标
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功能
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
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别名
- FL antibody
- Flt 3 ligand antibody
- Flt 3L antibody
see all
图片
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Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody (ab9688)
To detect human Flt3 ligand/Flt3L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84261 is required. This biotinylated polyclonal antibody, in conjunction with an anti-Human Flt3-ligand (ab9688) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Flt3 ligand/Flt3L.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (3)
ab9688 被引用在 3 文献中.
- Yuan X et al. Mesenchymal stem cell therapy induces FLT3L and CD1c+ dendritic cells in systemic lupus erythematosus patients. Nat Commun 10:2498 (2019). PubMed: 31175312
- Barry KC et al. A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments. Nat Med 24:1178-1191 (2018). PubMed: 29942093
- Patel ES et al. Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation. BMC Immunol 13:46 (2012). Flow Cyt ; Human . PubMed: 22897934
