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AB259726

重组Anti-Flt3 ligand/Flt3L抗体[EPR22288-201] - BSA and Azide free (Detector)

Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Flt3 ligand/Flt3L antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG

1 Images
Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] - BSA and Azide free (Detector) (AB259726)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] - BSA and Azide free (Detector) (AB259726)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab254507).

不同偶联物与剂型 (1)

  • Biotin

    Biotin Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] (Detector)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22288-201

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB254507

Human Flt-3 ligand ELISA Kit

Recombinant|SimpleStep

ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

  • 0
  • 0
Sample Prep & Detection Kits

AB201799

DyLight® 488 Conjugation Kit (Fast) - Lightning-Link®

Flow Cytometry - DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (AB201799)

  • 5
  • 1
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

  • 0
  • 0
Primary Antibodies

AB285002

Anti-IL37 antibody [EPR24011-134] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL37 antibody [EPR24011-134] - BSA and Azide free (Capture) (AB285002)

  • 0
  • 0
Primary Antibodies

AB284998

Anti-IL-32 antibody [EPR23983-43] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-32 antibody [EPR23983-43] - BSA and Azide free (Capture) (AB284998)

  • 0
  • 0
Primary Antibodies

AB307118

Anti-Cystatin D antibody [EPR25935-44] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Cystatin D antibody [EPR25935-44] - BSA and Azide free (Detector) (AB307118)

  • 0
  • 0
Primary Antibodies

AB244843

Anti-IL-31 antibody [EPR20205-211] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-31 antibody [EPR20205-211] - BSA and Azide free (Capture) (AB244843)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab259726 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Flt-3 ligand. The recommended pair for sandwich ELISA is:
Capture: ab259725, Human Flt-3 ligand Capture Antibody (unconjugated)
Detector: ab259726, Human Flt-3 ligand Detector Antibody (unconjugated)
The reference range value is 4.7 - 300 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).
See full target information FLT3LG
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